Cells or tissue coated with non-fibrogenic alginate less than 20

Details Cells or tissue coated with non-fibrogenic alginate less than 20 Cells or tissue coated with non-fibrogenic alginate less than 20

1995-03-14

1997-08-12

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

4351731, 435174, 435182, C12N 1110, C12N 1100, C12N 1104, C12N 1300

Patent

active

056564686

ABSTRACT:
An electrostatic process is used for coating a biological material with a uniform, continuous polymer layer by discharging a suspension of the biological material in a gelable coating polymer solution in a continuous stream through an orifice into an electrostatic field. The stream is attenuated to form droplets by maintaining an electrostatic voltage between the needle and the gelling solution which is sufficient to maintain an attraction of at least 1.times.10.sup.-6 newtons on the stream of liquid leaving the needle, and the droplets are collected in a gelling solution. A preferred product is pancreatic islets having a continuous, smooth coating of high polymannuronate non-fibrogenic alginate having a thickness less than 200 .mu.m such as about 20-200 .mu.m. The alginate preferably contains less than 1 wt. % fucose, less than 0.5 wt. % sulfate and less than 0.01 wt. % phloroglucinol, is free of fibrinoogenic concentration of protein, and has a mannuronate to guluronate ratio of from 1.2 to 6. Preferably, the electrostatic field is formed by applying an electrostatic continuous DC voltage to each the needle and gelling solution so that the needle and solution have opposite charges.

REFERENCES:
patent: 4744933 (1988-05-01), Rha et al.
patent: 4749620 (1988-06-01), Rha et al.
patent: 4789550 (1988-12-01), Hommel et al.
patent: 5429821 (1995-07-01), Dorian et al.

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