Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-05-31
1996-12-31
Stone, Jacqueline M.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352401, 4351721, C12N 1500, C12N 500
Patent
active
055893694
ABSTRACT:
Homozygotic cells are obtained by employing homologous recombination with a construct comprising a marker gene. The marker gene allows for selection without amplification and by employing elevated levels of the antibiotic to which the marker gene imparts resistance, gene conversion can occur, where in a diploid host, both copies of the target locus will be the same. In this manner, knock-outs of genes can be readily achieved without requiring two steps of homologous recombination.
REFERENCES:
Zijlstra et al (1989) Nature 342, 435-438.
Blomberg et al (1982) Proced. Natl. Acad. Sci. 79, 530-533.
Paluean et al (1989) Gene 85, 421-426.
Campbell and Worton (1981) Mo. Cell. Bio. 1:336-346. Segregation of recessive phenotypes on somatic cell hybrids: Role of mitoic recombination, gene inactivation and chromosomal non-disjunction.
T. Rajan, et al. (1990) J. Imm. 145:1598-1602. Rate and mechanism of generation of .beta.2-microglobulin mutants from a heterozygous murine cell line.
T. Potter, et al. (1987) P.N.A.S. 84:1634-1637. Mitotic recombination between homologous chromosomes generates H-2 somatic cell variants in vitro.
F. Nelson, et al. (1989) M. C. Bio. 9:1284-1288. Mitotic recombination is responsible for the loss of heterozygosity in cultured murine cell lines.
K. Paludin, et al. (1989) Gene 85:421-426. Gradauted resistance to G418 leads to differential selection of culture mammalian cells expressing the neo gene.
R. Mortenson, et al. (1991) P.N.A.S. 88:7036-7040. Embryonic stem cells lacking a functional inhibitory G-protein subunit produced by gene targeting of both alleles.
Jakobovits Aya
Seidman Jonathan G.
Cell Genesys Inc.
Crouch Deborah
Krupen Karen I.
Stone Jacqueline M.
The President and Fellows of Harvard College
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