Cells expressing fusion proteins of immunoglobulins

Details Cells expressing fusion proteins of immunoglobulins Cells expressing fusion proteins of immunoglobulins

2005-01-04

2005-01-04

Wilson, Michael C. (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C424S093100, C424S093200, C424S093210

Reexamination Certificate

active

06838281

ABSTRACT:
The invention provides methods and compositions for inducing and maintaining tolerance to epitopes or antigens containing the epitopes. The compositions include expression cassettes and vectors including DNA sequences coding for a fusion immunoglobulin operably linked to transcriptional and translational control regions functional in a hemopoietic or lymphoid cell. The fusion immunoglobulin includes at least one heterologous tolerogenic epitope at the N-terminus variable region of the immunoglobulin. Cells stably transformed with the expression vector are formed and used to produce fusion immunoglobulin. The invention also provides methods for screening for novel tolerogenic epitopes and for inducing and maintaining tolerance. The methods of the invention are useful in the diagnosis and treatment of autoimmune or allergic immune responses.

REFERENCES:
patent: 5167956 (1992-12-01), Neville et al.
patent: 5508386 (1996-04-01), Zanetti et al.
patent: 5817308 (1998-10-01), Scott et al.
patent: 6248332 (2001-06-01), Romet-Lemonne et al.
patent: 6258358 (2001-07-01), Romet-Lemonne et al.
patent: WO 9009804 (1990-09-01), None
patent: WO 9206193 (1992-04-01), None
Zambidis et al. J. Cellular Biochem., vol. O, No. 17, Part B, p. 251, 1993.*
Zanetti et al. Nature, vol. 355, pp. 476-477, Jan. 30, 1992.*
Chambers et al. PNAS, USA, vol. 89, pp. 1026-1030, Feb. 1992.*
Zambidis et al. Genetically transferred central and peripheral immune tolerance via retroviral-mediated expression iof immunogenic epitopes in hematopoietic progenitors or peripheral B lymphiocytes pp. 212-224 vol. 3, No. 3 1997.*
Porto et al. A soluble divalent class I major histocompatibility complex molecule inhibits alloreactive T cells at nanomolar concentrations pp. 6671-6675 1993.*
Vie et al. Human fusion proteins between interleukin 2 and IgM heavy chain are cytotoxic for cells expressing the interleukin 2 receptor vol. 89 pp. 11337-11341 1992.*
Arulanandam et al. A soluable multimeric recombinanr CD2 protein identifies CD48 as a low affiniry ligand for human CD2: divergence of CD2 ligands during the evolution of humans and mice pp. 1439-1450 vol. 177 1993.*
Sekigawa CD4 immunoadhesin, but not recombinant soluble CD4, blocks synctium formation by human immunodeficiency virus type 2-infected lymphoid cells pp. 5194-5198 1990.*
Zwirner et al. Expression of a functional chmeric Ig-MHC class II protein1 pp. 272-276 No. 1 1992.*
Zambidis Epitode-specific tolerance induction with an engineered immunoglobulin pp. 5019-5024 1996.*
Agarwal et al. Retroviral gene therapy with an immunoglobulin-antigen fusion construct protects from experimental autoimmune uveitis vol. 106 No. 2 2000.*
Kang et al. Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness pp. 8609-8614 1999.*
Kuby Immunlolgy p. 117 1994.*
McDonnell et al. Cell, Apr. 7, 1989, vol. 57. p. 79-88.*
Definition of “alllergen”.*
Nishijima 1997, Blood. vol. 90. p. 1031-1038.*
Ballard et al., “Mutational analysis of the immunoglobulin heavy chain promoter region”,PNAS USA, 83,9626 (1986).
Barbas et al., “Assembly of combinatorial antibody libraries on phage surfaces: The gene III site”,PNAS USA, 88,7978 (1991).
Bond et al., “Multiple Amb a I Allergens Demonstrate Specific Reactivity with IgE and T Cells from Ragweed-allergic Patients”,J. Immunol., 1146,3380 (1991).
Borel, “Haptens Bound to Self IgG Induce Immunologic tolerance; While When Coupled to Syngeneic Spleen Cells They Induce Immune Suppression”,Immunologic Reviews, 50,71 (1980).
Chambers et al., “Ectopic lymphokine gene expression in human peripheral blood lymphocytes in vivo”, PNAS USA, 89, 1026 (1992).
“Partial Digestion of DNA with Restriction Endonucleases”, inCurrent Protocols in Molecular Biology,Ausubel et al., eds., J. Wiley & Sons: New York, NY; vol. 1, Supplement 3.1.3; (1987).
“Enzymatic Manipulation of DNA and RNA” inCurrent Protocols in Molecular Biology,Ausubel et al., eds., J. Wiley/Green Publishing Associates; New York, NY, Chapter 3, pp. 3-1 to 3-44; (1992).
Gaur et al., “B Cell Tolerance Induction by Cross-Linking of Membrane IgM, but not IgD, and Synergy by Cross-Linking of Both Isotypes”,Journal of Immunology, 150,1663 (1993).
Hebell et al., “Suppression of the Immune Response by a Soluble Complement Receptor of B Lymphocytes”,Science, 254,102 (1991).
Huse et al., “Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda,”Science, 246,1275 (1989).
Jennings et al., “Fimbriae ofBacteriodes nodosus:protein engineering of the structural subunit for the production of an exogenous peptide”,Protein Eng., 2,365 (1989).
Kang et al., “Long-term expression of a T-Cell receptor β-chain gene in mice reconstituted with retrovirus-infected hematopoietic stem cells,”PNAS USA, 87,9803 (1990).
Kuo et al., Purification and Immunochemical Characterization of Recombinant and Native Ragweed Allergen amb a II,Molecular Immunol., 30,1077 (1993).
Lai et al., “T Cell Receptor Gene Usage in the Response to λ Repressor cI Protein, An Apparent Bias in the Usage of a Vα Gene Element,”J. Exp. Med., 168,,1081 (1988).
Lanza et al., “Use of Antigenized Antibodies Containing CD4 Sequences to Generate Antibodies Able to Inhibit Synctia Formation,”FASEB Journal, 6;A1400, Abstract No. 2690 (1992).
Olson et al., “Two Major Human Allergenic Sites on Ragweed Pollen Allergen Antigen E Identified by Using Monoclonal Antibodies,”J. Immunol., 136,2109 (1986).
Rafnar et al., Cloning of Amb α I (Antigen E), the Major Allergen Family of Short Ragweed Pollen,J. Biol. Chem., 266,1229 (1991).
F. Ria et al., “Immunological activity of covalently linked T-cell epitopes,”Nature, 343,381 (1990).
Rossi et al., “Adhesive Functions of Antibodies Antigenized with the Arg Cly Asp (RGD) Epitope”,FASEB Journal, 6,A1400, Abstract No. 2691 (1992).
Rutgers et al., “Hepatitis B Surface Antigen as Carrier Matrix for the Repetitive Epitope of the Circumsporozoite Protein ofPlasmodium Flaciparum,” Biotechnology, 6,1065 (1988).
Scherer et al., “Control of Cellular and Humoral Immune Responses by Peptides Containing T-Cell Epitopes”, inSymp. on Quant. Biol.;Cold Spring Harbor, New York, NY, vol. 54, pp. 2497-2504 (1989).
Scott, Cellular Events in Tolerance—V. Detection, Isolation and Fate of Lymphoid Cells Which Bind Fluoresceinated Antigen in Vivo,Cell. Immunol., 22,311 (1976).
Scott, “Multiple Pathways of B Lymphocyte Tolerance,”Immunol. Rev., 43,241 (1979).
Smith, “Immunological Tolerance of Nonliving Antigens”,Advances in Immunology, 1,67 (1961).
Venkataraman, M., et al., “Persistence of Antigen-Binding Cells with Surface Toleragen: Isologous Versus Heterologous Immunoglobulin Carriers”,J. Immunol., 119,1006-1009 (1977).
Warner et al., “A Polyclonal Model for B Cell Tolerance—I. Fc-Dependent Induction of Nonresponsiveness by Pretreatment of Normal Splenic B Cells with Anti-Ig,”J. Immunol., 146,2185 (1991).
Zanetti et al., Antigenized Antibodies,Nature, 355,476 (1992).

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