Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-05
1997-05-20
Campell, Bruce R.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 435 6, 935 22, 935 56, C12N 500, C12N 1500
Patent
active
056311532
ABSTRACT:
Positive-negative selector (PNS) vectors are provided for modifying a target DNA sequence contained in the genome of a target cell capable of homologous recombination. The vector comprises a first DNA sequence which contains at least one sequence portion which is substantially homologous to a portion of a first region of a target DNA sequence. The vector also includes a second DNA sequence containing at least one sequence portion which is substantially homologous to another portion of a second region of a target DNA sequence. A third DNA sequence is positioned between the first and second DNA sequences and encodes a positive selection marker which when expressed is functional in the target cell in which the vector is used. A fourth DNA sequence encoding a negative selection marker, also functional in the target cell, is positioned 5' to the first or 3' to the second DNA sequence and is substantially incapable of homologous recombination with the target DNA sequence. The invention also includes transformed cells containing at least one predetermined modification of a target DNA sequence contained in the genome of the cell. In addition, the invention includes organisms such as non-human transgenic animals and plants which contain cells having predetermined modifications of a target DNA sequence in the genome of the organism.
REFERENCES:
patent: 5464764 (1995-11-01), Capecchi et al.
Capecchi (1989) Science 244: 1288-1292.
Capecchi (1989) Trends in Genetics 5: 70-76.
Song et al (1987) Proc Natl Acad Sci USA 84: 6820-6824.
Mansour et al (1988) Nature 336: 348-352.
Paszkowski et al (1988) EMBO J 7: 4021-4026.
Thomas et al (1986) Cell 44: 419-428.
Wake et al (1985) Mol Cell Biol 5: 2080-2089.
Capecchi Mario R.
Thomas Kirk R.
Campell Bruce R.
University of Utah
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