Cellophane agar medium, and method for observing a microbe

Details Cellophane agar medium, and method for observing a microbe Cellophane agar medium, and method for observing a microbe

2005-09-13

2005-09-13

Naff, David M. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

C435S294100, C435S252100, C435S255700

Reexamination Certificate

active

06943005

ABSTRACT:
A cellophane agar medium is made of a cellophane piece with groove portions on at least one surface thereof and a nutritious agar formed on the surface of the cellophane piece via the groove portions. Then, the cellophane agar medium is disposed under wet condition, and a microbe is inoculated and cultivated on the cellophane agar medium. A fixing solution is added to the cellophane agar medium to fix the microbe to the cellophane agar medium. Thereafter, a given sample is made of the cellophane agar medium with the microbe and then, observed with a scanning electron microscope or a transmission electron microscope.

REFERENCES:
A. Ichinose et al.; “An Agar-Coated Cellophane Method for Bacterial Observation Using Scanning and Transmission Electron Microscopy”; Journal of Electron Microscopy Technology for Medicine and Biology; vol. 16, No. 3; 2002; pp 15-20.
Hiroshi Kushida; “New Methods for embedding with low viscosity epoxy resine”; The Cell, 13 (6); 1981; pp 184-188.
Hiroshi Kushida; “An Improved Embedding Method using ERL 4206 and Quetol 653”, J. Electron Microsc., vol. 29; No. 2; 1980; pp 193-194.
Kazunobu Amako et al.; “An Improved Method for Observation of Bacterial Growth Using the Scanning Electron Microscope”; J. Electro Microsc., vol. 26, No. 2; 1977; pp 155-159.
Motoyuki Matsuguchi et al.; “Optimal Condition for Observing Bacterial Flagella by the Scanning Electron Microscope”; J. Electro Microsc.; vol. 26, No. 4; 1977; pp 343-344.
Akitoyo Ichinose et al.; Method to observe active interaction between bacteria and cultured cell line; Re evaluation of processing specimen for electron microscope with cellophane film; The Cell; 29, (7); 1997; pp 278-281.
Kenji Tanaka; “Freeze Substitution Method for Microorganisms. II. Fungal Cell”; vol. 21, No. 1; 1986; pp 23-29.

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