Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1996-05-03
1999-03-30
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 72, 435 731, 435 732, 435 791-7.95, 435 97, 435 14, 435 4, 435810, 435 692, 435100, 435101, 435105, 435183, 435223, 435224, 435329, 514 1, 514 23, 514 54, 514 55, 436 89, 436 92, 436 94, 530370, 530371, 5303871, 5303875, C12Q 148, C12Q 100, G01N 3353
Patent
active
058887571
ABSTRACT:
Disclosed are methods that can be used to (1) measure the level of polysaccharide in a sample; (2) measure the ability of a compound to degrade a polysaccharide; (3) measure the ability of a compound to modulate polysaccharide synthesis; and (4) identify or distinguish a polysaccharide, and hence organism, for diagnostic purposes in clinical medicine or research. The invention stems from Applicant's discovery that polysaccharides have multiple binding sites for polysaccharide binding moieties (PBM, e.g., wheat germ agglutinin (WGA)). In each method, one PBM links the polysaccharide to a substrate, and a tagged PBM is used to detect the polysaccharide. All of these methods can be carried out rapidly and quickly in the wells of a microtiter plate, thus permitting high through-put screening of samples or test compounds.
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Hutzell Paula K.
Masood Khalid
Millennium Pharmaceuticals Inc.
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