Cell wall anchor proteins derived from yeast, genes thereof...

Details Cell wall anchor proteins derived from yeast, genes thereof... Cell wall anchor proteins derived from yeast, genes thereof...

2006-11-07

2006-11-07

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S004000, C435S006120, C435S320100, C435S069100, C435S071100, C435S254100, C435S440000, C530S350000, C536S023700, C536S023200

Reexamination Certificate

active

07132273

ABSTRACT:
The present invention provides novel cell wall anchor proteins derived from yeast, genes thereof and the genetic method for tethering polypeptide to the yeast cell wall using the same. Particularly, the present invention provides the novel GPI (glycosyl phosphatidyl inositol)-anchor protein genes, SFD1, GAS1, TIP1 and CWP1, and their proteins, PIR2 cell wall protein gene and its protein derived fromHansenula polymorpha, and the cell surface expression system using them which immobilize foreign enzymes or polypeptides on the cell wall of a microbial cell. In addition, the present invention provides the cell surface expression system using WSC1 gene and its protein derived from yeasts, includingHansenula polymorphaandSaccharomyces cerevisiae, and STA1 gene and its protein derived fromSaccharomyces diastaticus. The cell surface expression system of the present invention expects an immobilization effect as biocatalysts by adhereing a desired protein to the cell surface, and provides a means of altering target protein characteristics such as binding affinity and stability by library screening.

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