Chemistry: molecular biology and microbiology – Apparatus – Microorganism preservation – storage – or transport apparatus
Patent
1995-06-07
1998-07-07
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Microorganism preservation, storage, or transport apparatus
4352972, 4352975, 435 2, 435 13, C12M 306, C12M 112
Patent
active
057767696
ABSTRACT:
A mathematical model, and membranes and devices based upon that model, to optimize protocols for the addition or removal of cryoprotectant to or from biological cells. This disclosure describes the use of four equations to predict optimal protocols to add or remove cryoprotectant to or from biological cells. The equations particularly require experimentally found data, specific to cell-type and species, regarding the osmotic tolerance of the cells, where osmotic tolerance refers to the cells ability to shrink or swell to various changes in osmolality without injury. The equations further require the cryoprotectant permeability coefficient and the water permeability coefficient of the particular cells' plasma membrane. These coefficients are found with experimental data of the knetic volume change of the cell-type to a known concentration and temperature of cryoprotectant. Also disclosed are particularly preferred methods and devices to add or remove cryoprotectant to or from these cells based upon these equations.
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Critser John K.
Demeter Robert J.
Gao D. Y.
Beisner William H.
Methodist Hospital of Indiana
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