Cell transfection array for introduction of nucleic acid

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S325000, C435S375000

Reexamination Certificate

active

08029990

ABSTRACT:
The subject of the present invention is to provide a microarray for introducing nucleic acid, the microarray capable of introducing and expressing nucleic acid into cells simply by adding the nucleic acid onto a plate and the like, and then seeding the cells thereon and culturing them without adding a nucleic acid-introducing reagent or additives. The subject is achieved by preparing the microarray including atelocollagen, a gene-introducing agent and nucleic acid on a plate and the like for the introduction of nucleic acid. The nucleic acid can be introduced into a cell by seeding cells into which nucleic acids are introduced on the microarray and culturing them without the need of preparing a mixture of viral vectors, nucleic acids and a nucleic acid-introducing agent after culturing cells or the need of adding a nucleic acid-introducing agent and additives.

REFERENCES:
patent: 6998268 (2006-02-01), Terada et al.
patent: 2004/0266004 (2004-12-01), Terada et al.
patent: WO 03/000297 (2003-01-01), None
Ochiya et al. Biomaterials for Gene Delivery: Atelocollagen-mediated controlled release of molecular medicines. Current Gene Therapy 2001, vol. 1:31-52.
Steve Bailey, et al., “Applications of transfected cell microarrays in high-throughput drug discovery” Drug Dicove. Today., 2002, vol. 7 S113-118.
Cohen-Sacks et al., “Delivery and expression of pDNA embedded in collagen matrices” J. Control Release, 2004, vol. 95, pp. 309 to 320.
Vancha et al., “Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer,” BMC Biotechnol., 2004, vol. 4, pp. 23.
Homka et al., “The role of atelocollagen-based cell transfection array in high-throughput screening of gene functions and in drug discovery,” Current Drug Discovery Technologies, Bentham Science Publishers, NL, 1 (4):287-294, Dec. 1, 2004.
Junald Ziauddin and David M. Sabatini, “Microarrays of cells expressing defined cDNAs,” Nature 411, 107, May 2001.
Honma et al, “Atelocollagen-based Gene Transfer in Cells Allows High-Throughput Screening of Gene Functions,” Biochemical and Biophysical Research Communications 289, 1075-1081 (2001).
Takeshita and Ochiya, “Atelocollagen-mediated controlled release of molecular medicines,” Molecular Medicine, Feb. 2005, vol. 42, pp. 292 to 297, Abstract only.

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