Cell/tissue culture apparatus

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

Reexamination Certificate

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Details

C435S293100, C435S302100, C623S915000, C623S920000

Reexamination Certificate

active

07547540

ABSTRACT:
A cell/tissue culture apparatus for applying a desired tensile stress to a material to be cultivated such as a cell or tissue as a physical stimulation, thereby enhancing acceleration of culture of the material to be cultivated. The cell/tissue culture apparatus comprises a chamber (a culture chamber8) for circulating a culture fluid (24), a material to be cultivated (a matrix32) that is placed and cultivated in the chamber, stress generation means (an electromagnetic stretcher38, an actuator56) for causing a tensile stress to act on the material to be cultivated from an outside of the chamber, and control means (controllers50, 60) for causing the tensile stress generated by the stress generation means to intermit as well as undergo gradual increase or gradual decrease. The material to be cultivated can be caused to undergo expansion or contraction by applying and relieving the tensile stress, thereby applying a physical stimulation necessary for the proliferation to the material to be cultivated.

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Liu et al.‘Bio-Stretch, A computerized cell strain apparatus for three-dimensional organotypic cultures.’ In Vitro Cell. Dev. Biol.-Animal. vol. 35 (Feb. 1999), pp. 87-93.
International Search Report dated Nov. 1, 2002, International Appl. No. PCT/JP02/08753, 3 pages.
Supplemental European search report issued in corresponding European Patent Application No. 02762902.1 dated Jul. 3, 2008.

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