Cell test for detecting human chorionic gonadotropin

Details Cell test for detecting human chorionic gonadotropin Cell test for detecting human chorionic gonadotropin

1976-04-19

1979-03-13

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

1951035A, 424 8, 424 12, G01N 3316

Patent

active

041440315

ABSTRACT:
A diagnostic cell test and kit for detecting human chorionic gonadotropin as evidence of cancer. The subject's cells undergoing examination are contacted with artiserum specific to the beta-subunit of human chorionic gonadotropin, the antiserum having been raised in a host animal. The treated cells are divided into two portions. The portions are treated with antiserum to the gamma globulin of the host animal. The antiserum to the gamma globulin used in treating the first portion is conjugated to fluorescein and the antiserum used in contacting the second portion is conjugated to peroxidase. A positive test for cancer is concluded only when both tests on the two portions of the cells are positive.

REFERENCES:
patent: 3516794 (1970-06-01), Murty
patent: 3654090 (1972-04-01), Schuurs
patent: 3697638 (1972-10-01), Hansen
patent: 3720760 (1973-03-01), Bennich
patent: 3789116 (1974-01-01), Kay
patent: 3868219 (1975-02-01), Hurenkamp
patent: 3873683 (1975-03-01), Fishbein
patent: 3901654 (1975-08-01), Gross
patent: 3935074 (1976-01-01), Rubenstein
patent: 3992514 (1976-11-01), Donini
Chemical Abstracts I, 80:2073b, (1974).
Chemical Abstracts II, 81:985x, (1974).
J. J. Munoz, Anal. Chem., 31(6), 981-985, (1959).

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