Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1991-12-12
1993-04-06
Hill, Jr., Robert J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530403, C07K 1300
Patent
active
052005089
ABSTRACT:
We disclose the discovery and purification of a tumor antigen that binds the mouse monoclonal antibody L6. Immunohistochemical analysis showed that the antigen is expressed on the membrane surface of LX-1 human lung tumor cells, which retain L6 binding activity when intracerebrally xenografted in nude rats. The antigen is also expressed in the cytoplasm of hypothalamic neurons. Inability of oxytocin and vasopressin hormones to block L6 binding showed that the antigenic epitope resides in neurophysin, the carrier protein associated with the two hormones. Porcine neurophysin did block L6 binding to the antigen. Western blot analysis confirmed that L6-immunoreactivity is neurophysin-related. Immunoaffinity chromatography and gel electrophoresis revealed the antigen molecular weight to be 45000 daltons. Amino-terminal sequencing revealed a 21-amino acid homology with the N-terminus of human pro-pressophysin.
REFERENCES:
Land et al., Nature 295:299-303 (1982).
Breslow, Ann. Rev. Biochem. 48:251-274 (1979).
Hellstrom et al. Proc. Nat'l Acad Sci USA 83:7059-63 (1986).
Schlesinger et al. Proc Natl Acad Sci USA 69(11): 3350-54 (1972).
Neuwelt Edward A.
Nilaver Gajanan
Rosenbaum Lawrence
Guest Shelly J.
Hill Jr. Robert J.
The State of Oregon acting by and through the State Board of Hig
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