Cell substrate electrical impedance sensor with multiple electro

Details Cell substrate electrical impedance sensor with multiple electro Cell substrate electrical impedance sensor with multiple electro

1991-08-08

1993-02-16

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435301, 435 33, 435 40, 324692, 324439, 204403, G01N 2702, C12M 134, C12M 120

Patent

active

051870967

ABSTRACT:
An apparatus and method for monitoring cell-substrate impedance comprises upper and lower substrates each containing strips of metal foil which cross to form well areas. Holes in the upper substrate expose an insulation layer between the upper and lower substrates. Smaller holes in the insulation layer expose the conductive material of the lower substrate. Cylindrical well walls are positioned around each of the holes in the upper substrate and define a cell medium containing well which is exposed to an annular area of conductive foil on the upper substrate, and a small area of conducive foil on the lower substrate. The areas of foil form large and small electrodes which are positioned in an array of wells which can be used to monitor the impedance of various cell cultures under various conditions. A switching device applies currents to the well electrodes and a monitoring device measures the impedance between the electrodes in each well. In this way, cell spreading and motility can be measured.

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Physica D 38 (1989) 128-133.
J. Imm. Methods, 127 (1990) 71-77.

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