Cell stretching method

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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Details

435 29, C12N 506, C12Q 102

Patent

active

053488792

ABSTRACT:
Disclosed is a method for imparting to a living culture of cells biaxial mechanical forces which approximate the mechanical forces to which cells are subject in vivo. The method utilizes a displacement applicator which may be actuated to contact and stretch a membrane having a living cell culture mounted thereon. Stretching of the membrane imparts biaxial mechanical forces to the cells. These forces may be uniformly applied to the cells, or they may be selectively nonuniformly applied.

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patent: 4839280 (1989-06-01), Banes
patent: 4851354 (1989-07-01), Winston et al.
patent: 4940853 (1990-07-01), Vandenburgh
Andersen et al., "A Device for the Application of Known Simulated Orthodontic Forces to Human Cells In Vitro, " J. Biomechanics, vol. 24, No. 7, pp. 649-654 (1991).
Gilbert et al., "Characterization of the Surface Strain Applied to Cyclically Stretched Cells In Vitro, "35th Annual Meeting, Orthopaedic Research Society, p. 249 (Feb. 7, 1989).
Hasegawa et al., "Mechanical Stretching Increases the Number of Cultured Bone Cells Synthesizing DNA and Alters Their Pattern of Protein Synthesis," Calcif. Tissue Int, vol. 37, pp. 431-436 (1985).

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