Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1994-01-07
1998-05-26
Low, Christopher S. F.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
536 231, 536 235, 4353201, 4351723, 43525233, 43525411, 435325, 435363, 935 9, 935 10, 935 29, 935 72, 935 69, 935 70, C12N 121, C12N 1512, C12N 1563
Patent
active
057563438
ABSTRACT:
The present invention relates to DNA sequence coding for part or all of the heat shock transcription factor or heat shock factor (HSF) proteins derived from humans and Drosophila, and the proteins encoded by these sequences.
The present invention also includes methods for detecting HSF in a biological sample. The presence of HSF in the nucleus of a cell can be detected with specific anti-HSF antibody reagents. The presence of such HSF proteins in the nucleus indicates a stressed condition including diseases. Furthermore, the presence of multimeric HSF in the crude or fractionated cell extract is indicative of a stressed state.
REFERENCES:
patent: 5137805 (1992-08-01), Kingston et al.
Abdella, P.M.; Smith, P.K.; and Royer, G.P. "A New Cleavable Reagent for Cross-linking and Reversible Immobilization of Proteins." Biochem. Biophys. Research Communications 87 (3): 734-742, 1979.
Burnett, W.N. "Western Blotting": Electrophoretic Transfer of Proteins from Sodium Dodicyl Sulfate-Poly Acrylamide Gels to Unmodified Nitrocellulose and Radiographic Detection with Antibody and Radioiodinated Protein A. Analytical Biochemistry 112:195-203, 1981.
Kingston, R.E.; Schuetz, T.J.; and Larin, Z. "Heat-Induceble Human Factor that Binds to a Human Hsp70 Promoter." Mol. Cell. Biol. 7(4): 1530-1534, 1987.
Larson, J.S.; Schuetz, T.J.; and Kingston, R.E. "Activation in vitro of Sequence-Specific DNA Binding by a Human Regulatory Factor", Nature 335: 372-375, 1988.
Perisic, O.; Xiao, H.; and Lis, J.T. "Stable Binding of Drosophila Heat Shock Factor to Head-to-Head and Tail-to-Tail Repeats of a Conserved 5 bp Recognition Unit." Cell 59:797-806, 1989.
Schuetz, T.J.; Gallo, G.J.; Sheldon, L.; and Tempst, P. "Isolation of a cDNA for HSF2: Evidence for Two Heat Shock Factors in Humans," Proc. Natl. Acad. Sci. USA 88: 6911-6915, 1991.
Sorger, P.K. and Nelson, H.C.M. "Trimerization of a Yeast Transcriptional Activator via a Coiled-Coil Motif." Cell 59: 807-813, 1989.
Andersson, L.O.; Borg, H.; and Mikaelsson, M. "Molecular Weight Estimations of Protein by Electrophoresis in Polyacrylamide Gels of Graded Porosity." FEBS Letters 20(2): 199-201, 1972.
Watson et al. 1987, in: Molecular Biology Of The Gene, Fourth Edtion, Benjamin/Cummings Publ. co., Menlo Park, CA p. 313.
Clos Joachim
Rabindran Sridhar
Westwood J. Timothy
Wu Carl
Low Christopher S. F.
The United States of America as represented by the Department of
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