Cell strainer

Details Cell strainer Cell strainer

1994-01-25

1997-01-14

Dawson, Robert A.

Liquid purification or separation

Filter

Handled

210474, 210484, 2104972, 422101, B01D 29085

Patent

active

055935877

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND ART

The present invention relates to an improved cell strainer, and more particularly to a cell strainer for removing impurities such as supporting tissues and spiculae from a suspension when such a suspension containing lymphocytes is to be prepared for immunological study purposes and lymphocyte to be used for cell fusion are collected in a test tube.
Gauze was conventionally used for collecting the cells noted above. However, the recovery rate of a filtrate lowered as a liquid to be filtered was absorbed by gauze, which readily absorbs water.


DISCLOSURE OF INVENTION

After extensive studies of filter materials other than gauze, the inventor of the present invention has developed a cell strainer made of nylon net having a pore size of 40-200 mm whose filter mesh is capable of removing impurities such as supporting tissues and spiculae from a suspension containing lymphocytes as a large volume of filtrate to be processed is not absorbed by a filter mesh, as the filter mesh itself does not absorb water.
The present invention is a cell strainer to be equipped at the top of a tube for removing impurities from a suspension of cells or tissues comprising: tube, section holding said filter, which receives and filters the above-mentioned suspension, of a nylon net having a pore size of 40-200 mm.
The structure of the body frame is not critical in the present invention.


BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a perspective view of a cell strainer and tube embodying the present invention;
FIG. 2 is a graph showing the difference between a cell strainer and gauze in their absorption of normal saline solution.


BEST MODE FOR CARRYING OUT THE INVENTION

FIG. 1 is a perspective view of a cell strainer and tube of the present invention whose embodiments are further illustrated in detail by the accompanying drawing in which:
cell strainer 1 has a flange 3 being held by the upper opening of a tube 2, a grip 4 externally projecting from said flange, a frame body 5 extending downward and housed in a tube below said flange, and a section holding said filter has a filter component 6 composed of nylon net having pore sizes range from 40 to 200 microns.
It is desirable for the lower end of a frame body extended below a section that holds a filter to have an outer diameter substantially corresponding to an inner diameter of a tube.
The uses of a cell strainer of the present invention are as follows. suspension is prepared from various organs. groups are obtained from cultured cells. collection of various residues on a strainer.


INDUSTRIAL APPLICABILITY

Cell suspension (sample) can be prepared from various organs and cultured cells using different methods, when a sample liquid is poured over a cell strainer placed on a tube such as Falcon (Trademark) 2070 (Blue Max), using KOMAGOME (Tradename) pippet. This makes it possible to obtain a higher recovery ratio and a single cell suspension.


Sample Application
spleen, thymus, tonsil, lymph node, etc.


EXAMPLE

As for the volumes given in Table 1. The volumes of normal saline solution absorbed by a cell strainer of this invention and gauze were studied.


TABLE 1 ______________________________________ 2 Sheets of Cell strainer 2 .times. 30 mesh gauze (200 mesh) (50 mesh) Re- Re- covered Re- covered Re- Liquid liquid covery liquid covery volume volume Mean ratio volume Mean ratio (ml) (ml) (ml) (%) (ml) (ml) (%) ______________________________________ 50 49.98 49.87 .+-. 99.7 .+-. 48.00 47.01 .+-. 94.02 .+-. 49.68 0.11 0.23 46.84 0.58 0.16 49.90 46.61 49.92 47.00 49.89 46.58 40 39.90 39.93 .+-. 99.8 .+-. 36.82 37.11 .+-. 92.78 .+-. 39.85 0.05 0.13 36.85 0.40 0.99 39.97 37.23 39.96 36.90 39.95 37.76 30 29.95 29.93 .+-. 99.8 .+-. 27.50 27.70 .+-. 92.33 .+-. 29.99 0.06 0.19 27.89 0.40 1.33 29.84 27.50 29.92 27.30 29.88 28.30 20 19.98 19.89 .+-. 99.5 .+-. 18.08 17.79 .+-. 88.95 .+-. 19.98 0.13 0.74 18.40 0.89 4.47 19.97 18.75 19.67 17.00 19.87 16.70 10 9.95 9.91 .+-. 99

REFERENCES:
patent: 2839056 (1958-06-01), Mailly
patent: 4131549 (1978-12-01), Ferrara

Affiliated with

Also associated with

Rating

Cell strainer does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell strainer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell strainer will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1385605