Cell-specific molecule and method for importing DNA into a nucle

Details Cell-specific molecule and method for importing DNA into a nucle Cell-specific molecule and method for importing DNA into a nucle

1997-11-05

2000-10-10

Priebe, Scott D.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

424 9321, 4353201, 435325, 435455, 435458, 536 231, 536 241, A61K 4800

Patent

active

061302074

ABSTRACT:
The invention provides a cell-specific nuclear targeting molecule having a nucleic acid sequence which includes a binding site for a nuclear DNA binding protein expressed only in a specific cell type. The invention further provides a plasmid for targeting a DNA molecule into the nuclei of a specific cell type. The plasmid comprises the cell-specific nuclear targeting molecule and a DNA molecule to be targeted to the nuclei of the specific cell type. This plasmid of the subject invention can be introduced into various host cells, and the cell-specific nuclear targeting molecule will target the DNA molecule to the nuclei of the specific cell type. Thus, the invention further provides a method of targeting a DNA molecule into the nuclei of a specific cell type. The method comprises providing a plasmid (the plasmid comprising the cell-specific nuclear targeting molecule and the DNA molecule to be targeted) and introducing the plasmid into the cytoplasm of the specific cell type. In this method, the cell-specific nuclear targeting molecule targets the DNA molecule into the nuclei of the specific cell type.

REFERENCES:
patent: 5786171 (1998-07-01), Lee et al.
patent: 5837283 (1998-11-01), McDonald et al.
patent: 5877020 (1999-03-01), Alitalo
patent: 5888765 (1999-03-01), Patterson et al.
patent: 5916763 (1999-06-01), Williams et al.
Szucsik et al., Genomics 28, 154-162, 1995.
Ishii, N., et al., J Virol 68(12):8209-8216 (Dec. 1994).
Feldherr, C., et al., Exp Cell Res 213(1):164-171 (Jul. 1994).
Dean, D.A., and Kasamatsu, H., J Biol Chem 269(7):4910-4916 (Feb. 18, 1994).
Xue, Z., et al., Eur J Cell Biol 62(1):13-21 (Oct. 1993).
Feldherr, C.M., et al., Proc Natl Acad Sci USA 89(22):11002-11005 (Nov. 15, 1992).
Yoneda, Y., et al., Exp Cell Res 201(2):313-320 (Aug. 1992).
Lassner, M.W., et al., Plant Mol Biol 17(2):229-234 (Aug. 1991).
Rihs, H.P., et al., EMBO J 10(3):633-639 (Mar. 1991).
Clever, J., and Kasamatsu, H., Virology 181(1):78-90 (Mar. 1991).
Ono, T., et al., Neurosci Lett 117(3):259-263 (Sep. 18, 1990).
Gharakhanian, E., and Kasamatsu, H., Virology 178(1):62-71 (Sep. 1990).
Lobl, T.J., et al., Biopolymers 29(1):197-203 (Jan. 1990).
Wychowski, C., et al., EMBO J 5(10):2569-2576 (Oct. 1986).
Graessman, M., et al., Nucl Acids Res 17(6):6603-6612 (1989).
Dean, D.A. Abstract #313 of the Keystone Symposia on Molecular and Cellular Biology, Taos, New Mexico, Feb. 4-10, 1996.
Dean, D.A., Exp Cell Res 230:293-302 (1997).
Dowty, M.E., et al., Proc Natl Acad Sci USA 92:4572-4576 (1995).
Nigg, E.A., Nature 386:779-787 (1997).
Zimmer, W.E., et al., Abstract, Develop Biol 175:399 (1996).

Affiliated with

Also associated with

Rating

Cell-specific molecule and method for importing DNA into a nucle does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell-specific molecule and method for importing DNA into a nucle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell-specific molecule and method for importing DNA into a nucle will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2257089