Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus
Reexamination Certificate
2003-04-23
2009-08-11
Beisner, William H (Department: 1797)
Chemistry: molecular biology and microbiology
Apparatus
Mutation or genetic engineering apparatus
C435S173100, C435S173400, C435S173500, C435S173800, C435S287200, C435S288300, C435S288400, C435S461000, C435S470000, C435S173600, C264S081000, C264S105000, C427S122000, C427S249600
Reexamination Certificate
active
07572623
ABSTRACT:
The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold, which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging.
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Eppich Henry
Mangano Joseph
Beisner William H
Bowers Nathan A
Wolf Greenfield & Sacks P.C.
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