Cell separation using electric fields

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target

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4351731, 4351734, 4351736, C12N 1300

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active

060430664

ABSTRACT:
The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold; which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging.

REFERENCES:
patent: 4714680 (1987-12-01), Civin
patent: 4822470 (1989-04-01), Chang
patent: 4965204 (1990-10-01), Civin
patent: 5048404 (1991-09-01), Bushnell et al.
patent: 5744347 (1998-04-01), Wagner et al.
patent: 5750397 (1998-05-01), Tsukamoto et al.
patent: B15035994 (1995-02-01), Civin
patent: B15130144 (1995-08-01), Civin
J. Audet et al., "Advances in hematopoietic stem cell culture", Current Opinion in Biotechnology, 9: 146-151, 1998.
J. Banchereau et al., "Long-Term Human B Cell Lines Dependent on Interleukin-4 and Antibody to CD40", Science, vol. 251, pp. 70-72, Jan. 1991.
A. Berardi et al., "Functional Isolation and Characterization of Human Hematopoietic Stem Cells", Science, vol. 267, pp. 104-108, Jan. 1995.
S.L. Berger, "Lymphocytes as Resting Cells", Methods in Enzymology, Chapter entitled Specific Cell Lines, vol. LVIII, Section 42, pp. 486-494, 1979.
T. Grahl et al., "Killing of microorganisms in fluid media by high-voltage pulses", DECHEMA Biotechnology Conferences 5, pp. 675-678, 1992.
W.A. Hamilton and A.J.H. Sale, "Effects of High Electric Fields on Microorganisms II. Mechamism of Action of the Lethal Effect", Bichimica et Biophysica Acta, BBA 25877, vol. 148, pp. 789-800, Jul. 1967.
H. Hulsheger and E.G. Niemann, "Lethal Effects of High-Voltage Pulses on E. Coli K12", Radiation and Environmental Biophysics, vol. 18, pp. 281-288, 1980.
H. Hulsheger et al., "Killing of Bacteria with Electric Pulses of High Field Strength", Radiation and Environmental Biophysics, vol. 20, pp. 53-65, 1981.
H. Hulsheger et al., "Electric Field Effects on Bacteria and Yeast Cells", Radiation and Environmental Biophysics, vol. 22 pp. 149-162, 1983.
K. Kinosita, Jr. and T.Y. Tsong, "Hemolysis of human erythrocytes by a transient electric field", Proc. Natl. Acad. Sci. USA, vol. 74, No. 5, pp. 1923-1927, May 1977.
K. Kinosita, Jr. and T.Y. Tsong, "Voltage-Induced Pore Formation and Hemolysis of Human Erythrocytes", Biochimica et Biophysica Acta, vol. 471, pp. 227-242, 1977.
J.F. Lechner et al., "Normal Human Prostate Epithelial Cell Cultures", Methods in Cell Biology, vol. 21B, Chapter 9, pp. 195-225, 1980.
S.B. Dev and G.A. Hoffmann, "Clinical Applications of Electroporation", Electrical Manipulation of Cells, P.T. Lynch and M.B. Davey editors, pp. 187-191, 1996.
A.J.H. Sale and W.A. Hamilton, "Effects of High Electric Fields on Microorganisms I, Killing of Bacteria and Yeasts", Biochimica et Biophysica Acta, BBA 25876, vol. 148, pp. 781-788, Jul. 1967.
A.J.H. Sale and W.A. Hamilton, "Effects of High Electric Fields on Micro-Organisms III, Lysis of Erythrocytes and Protoplasts", Biochimica et Biophysica Acta, BBA 75160, vol. 163, pp. 37-43, 1968.
T. Sato et al., "Establishment of a human leukaemic cell line (CMK) with megakaryocytic characteristics from a Down's syndrome patient with acute megakaryoblastic leukaemia", British Journal of Haematology, vol. 72, pp. 184-190, 1989.
O.F. Schanne and E.R.P.-Ceretti, Impedance Measurements in Biological Cells, Chapter 5.3, pp. 331-337, 1978.
S. Sixou and J. Teissie, "Specific electropermeabilization of leucocytes in a blood sample and application to large volumes of cells", Biochimica et Biophysica Acta, BBAMEM 75004, vol. 1028, pp. 154-160, 1990.
J. Teissie and T.Y. Tsong, "Electric Field Induced Transient Pores in Phospholipid Bilayer Vesicles", Biochemistry, vol. 20, No. 6, pp. 1548-1554, 1981.
T.Y. Tsong, "Electrically Stimulated Membrane Breakdown", Electrical Manipulation of Cells, P.T. Lynch and M.R. Davey editiors, Chapter 2, pp. 15-36, 1996.
A. Valle et al., "Activation of human B lymphocytes through CD40 and interleukin 4", Eur.J.Immunol., pp. 1463-1467, 1989.
D. Zipori and F. Lee, "Introduction of Interleukin-3 Gene Into Stromal Cells From the Bone Marrow Alters Hemopoietic Differentiation but Does not Modify Stem Cell Renewal", Blood, vol. 71, No. 3, pp. 586-596, Mar. 1988.
H. Berg et al., "Electric Field Effects on Biological Membranes: Electroincorporation and Electrofusion," Ettore Majorana Int. Sci. Ser.: Phys. Sci., vol. 32 (Bioelectrochem. 2: Membr. Phenom.), pp. 135-166, 1987.
L. Graziadei et al., "Introduction of Unlabeled Proteins into Living Cells by Electroporation and Isolation of Viable Protein-Loaded Cells Using Dextran-Fluorescein Isothiocyanate as a Marker for Protein Uptake," Analytical Biochemistry, vol. 194, pp. 198-203, 1991.
S. Mischke et al., "A versatile low-cost apparatus for cell electrofusion and other electrophysiological treatments," Journal fo Biochemical and Biophysical Methods; vol. 13, pp. 65-75, 1986.
G.H. Hofmann and G.A.Evans, "Electronic Genetic-Physical Biological Aspects of Cellular Electromanipulation," IEEE Engineering in Medicine and Biology Magazine, pp. 6-23, Dec. 1986.

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