Cell-releasing agent and method of releasing cell sheet

Details Cell-releasing agent and method of releasing cell sheet Cell-releasing agent and method of releasing cell sheet

2005-09-09

2010-11-09

Jiang, Shaojia Anna (Department: 1623)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

C560S169000, C536S046000

Reexamination Certificate

active

07829337

ABSTRACT:
A cell-sheet releasing agent of the present invention contains an aminated polyrotaxane. The polyrotaxane constituting the skeleton of the cell-releasing agent of the present invention has a structure in which cavities of a plurality of cyclic molecules are threaded onto a linear molecule and both terminals of the linear molecule have a bulky cap bonded thereto so that the cyclic molecules are not dethreaded from the linear molecule. Furthermore, the aminated polyrotaxane contained in the cell-releasing agent of the present invention is a compound in which at least some of hydroxy groups in the cyclodextrin structure contained in the polyrotaxane are each substituted with a substituent having an amino group. According to this cell-sheet releasing agent, cultured cells anchored to the surface of a container can be released without damaging the cells and without controlling the temperature.

REFERENCES:
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Ooya et al.; “Synthesis of theophyline-polyrotaxane conjugates and their drug release via supramolecular dissociation;”Journal of Controlled Release; vol. 58; 1999; pp. 251-269; Elsevier Science B.V.
Tomoyuki Furubayashi et al., “Hydrolyzability for optimization of cartilage tissue regeneration environment, Control of properties of polyrotaxane hydrogel,” The Society of Polymer Science, Japan, Summary of the Symposium on Macromolecules, 2003, vol. 32, pp. 45-46.

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