Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-07
1998-08-11
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 5, 435 6, 435 71, 435 72, 435 75, 435 79, 435 29, 435 34, 435839, 435834, 435848, 435878, 435881, 435883, 435966, 436527, 436531, 436807, 436808, 436813, G01N 33574
Patent
active
057926174
ABSTRACT:
A test kit and method for the highly sensitive detection of specific analytes in a sample is provided. The presence of the analyte in the sample results in a decrease in the concentration of a growth inhibiting substance leading to proliferation of cells in the region of the analyte. The presence or absence of the analyte is determined by detecting the presence of increased numbers of cells. Assay sensitivity is accounted for by the exponential amplification of cell number that occurs during cell proliferation in the presence of analyte.
REFERENCES:
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4665018 (1987-05-01), Vold
patent: 4670382 (1987-06-01), Buckley et al.
patent: 4880731 (1989-11-01), Kaspar et al.
patent: 5194382 (1993-03-01), Herrmann et al.
patent: 5244785 (1993-09-01), Loor et al.
patent: 5306468 (1994-04-01), Anderson et al.
patent: 5385822 (1995-01-01), Melnicoff et al.
patent: 5472846 (1995-12-01), Rotman
Hunter and Bottenstein, "Growth Factor Responses of Enriched Bipotential Glial Progenitors," 54, Developmental Brain Research (Amsterdam, the Netherlands) pp. 235-248 (1990).
Rotman and Cox, "Specific Detection of Antigen-Binding Cells by Localized Growth of Bacteria," 68, Proc. Nat. Acad. Sci. (Washington, D.C.) pp. 2377-2380 (Oct. 1971).
Wilchek and Bayer, "The Avidin-Biotin Complex in Bioanalytical Applications," 171, Analytical Biochemistry (San Diego, CA) pp. 1-32 (May 1988).
Knaack David
Spiegel Carol A.
LandOfFree
Cell proliferation-based amplified detection of analytes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cell proliferation-based amplified detection of analytes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell proliferation-based amplified detection of analytes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-387929