Cell preservative solution

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition

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436 18, 436 8, 436826, 435 1, 435 2, G01N 3100, A01N 102

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active

052565718

ABSTRACT:
An aqueous alcohol buffer solution for substantially ambient, in vitro preservation of mammalian cells for a selected duration. The solution generally contains a water-miscible alcohol in an amount sufficient to fix the sample cells without coagulation, an anti-clumping agent, and a buffer agent to maintain the solution at a pH within a range of four to seven.

REFERENCES:
patent: 4090977 (1978-05-01), Dubin
patent: 4390632 (1983-06-01), Carter, II
patent: 4493821 (1985-01-01), Harrison
WPI, File Supplier, Accession No. 74-81767V (47), Derwent Publications Ltd. London, GB; & JP-A-49 039 437 (Toa Tokushu Denki Co., Ltd) Oct. 25, 1974.
Maitland et al., "Freeze-substitution Staining of Rat Growth Plate Cartilage With Alcian Blue for Electron Microscopic Study of Proteoglycans.sup.1 ", The Journal of Histochemistry and Cytochemistry, vol. 37, No. 3, pp. 383-387, 1989.
Kurki et al., "Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry", Jour. of Immunological Methods, 109 (1988) pp. 49-59.
Campana et al.,. "Double and triple staining methods for studying the proliferative activity of human B an T lymphoid cells", Journal of Immunological Methods, 107 (1988) pp. 79-88.
Ronne, "Chromosome Preparation and High Resolution Banding Techniques. A Review.", Symposium: Cytogenetics and Cell Biology, J. Dairy Sci., 72:1363-1377, (1989).
Levitt et al., "Methanol fixation permits flow cytometric analysis of immunofluorescent stained intracellular antigens", Journal of Immunological Methods, 96 (1987) 233-237.
Gill et al., "Laboratory Cytophathology Techniques For Specimen Preparation", The Johns Hopkins Hospital, Sixth Edition, 1980, pp. 3-1-3-3.
Pearson et al., "Evaluation of Collection and Preservation Techniques for Urinary Cytology", Acta Cytologica, 25(3): (May-Jun. 1981), pp. 327-333.
Tsuchihashi et al., "Quantification of Nuclear DNA and Intracellular Glycogen in a Single Cell by Fluorescent Double-Staining", Histochemistry, 63. 311-322 (1979).
Koss, Diagnostic Cytology and Its Histopathologic Bases, vol. Two, pp. 1187-1202.
Keebler et al., A Manual of Cytotechnology, Sixth Edition, American Society of Clinical Pathologists Press, 1983, pp. 321-322.
Villanueva, A. R., J. Histotechnology, 9(3). 1986. pp. 155-161 (Biosis Abstract).
Rost et al, Histochem. J., 7(4), Jul. 1975, pp. 307-320 (Biosis Abstract).
Baumgaertner et al, J. Clin. Microbiol., 26(10) 1988 pp. 2044-2047 (Biosis Abstract).
Kehr et al, Biologia, 39(11), 1984) p. 1107-1114 (Biosis Abstract).

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