Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2008-01-15
2008-01-15
Siew, Jeffrey (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S071100, C530S412000
Reexamination Certificate
active
07319021
ABSTRACT:
Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
REFERENCES:
patent: 4797474 (1989-01-01), Patroni et al.
patent: 4992531 (1991-02-01), Patroni et al.
patent: 5489676 (1996-02-01), Elsbach et al.
patent: 5593866 (1997-01-01), Hancock et al.
patent: 5760189 (1998-06-01), Vicik et al.
patent: 5973137 (1999-10-01), Heath
patent: 5981235 (1999-11-01), Shultz et al.
patent: 6174704 (2001-01-01), Chu et al.
patent: 6210915 (2001-04-01), Shay et al.
patent: 6242235 (2001-06-01), Shultz et al.
patent: 2001/0034066 (2001-10-01), Alam
patent: 2002/0012982 (2002-01-01), Blakesley et al.
patent: 2003/0054435 (2003-03-01), Grabski
patent: 0 173 937 (1986-03-01), None
patent: 0 222 725 (1992-03-01), None
patent: 0 671 473 (1995-09-01), None
patent: WO 01/00668 (2001-01-01), None
patent: WO 01/75147 (2001-10-01), None
patent: WO 02/06456 (2002-01-01), None
patent: WO 02/057478 (2002-07-01), None
Product Description for n-Octyl-b-D-thioglucoside, http://www.dojindo.com
ewprod/1/cell-m/detergen/8sglu.html, 2003.
Novagen, Inc. Product Description for BugBuster® Extraction Reagent, 2002.
Novagen, Inc. Product Description for His⊖Bind® Kits, 2002.
Pierce Product Description and Instructions for B-PER® Bacterial Protein Extraction Reagent, 2001.
Sigma-Aldrich Co. Product Description for Octyl-b-D-1-thioglucopyranoside, http://www.sigmaaldrich.com/cgi-bin/hsrun/Distributed/Hahtshop/HahtShop.htx;start=frm, 2003.
Tomah Products, Inc. Product Description For Ethoxylated Amines, 1998.
Fox, Charles, “Rationale For the Selection of Emulsifying Agents”,Cosmetics&Toiletries, vol. 101, 1986, pp. 25-44.
Graciaa, A., et al., “A Study of the Required Hydrophile-Lipophile Balance for Emulsification”,Landmuir, vol. 5, 1989, pp. 1315-1318.
Griffin, William C., “Calculation of HLB Values of Non-Ionic Surfactants”,Journal of the Society of Cosmetic Chemists, vol. 5, 1954, pp. 249-256.
Griffin, William C., “Classification of Surface-Active Agents by HLB”,Journal of the Society of Cosmetic Chemists, vol. 1, 1949, pp. 311-326.
Mörbe, et al., “Release of Miniantibodies fromE. coliCells into the Supernatant at Low and High Cell Densities,”Microbiological Research, vol. 152, 1997, pp. 385-394.
Berg B. Hofsten AV., “The Ultrastructure of the Cellulolytic Bacterium Cellvibrio Fulvus.”,Can. J. Microbiol., Mar. 1975, vol. 21, No. 3, p. 386-391. (Abstract Only).
Hancock, R.E.W., “Alterations In Outer Membrane Permeability”,Ann. Rev. Microbiol., 1984, vol. 38, p. 237-264.
Lin, Jung-Hsin et al., “Stability of a Melittin Pore in a Lipid Bilayer: A Molecular Dynamics Study”,Biophysical Journal, Apr. 2000, vol. 78, p. 1714-1724.
Grabski, et al., “Automated Methods for Solubility Screening and Recombinant Protein Purification”,American Biotechnology Laboratory, pp. 34, 36 and 38 (Aug. 2003).
Grabski et al., “Automated purification of recombinant proteins in a 96-well format with RoboPop™ Kits and robotic sample processing”,Innovations Newsletter, No. 14, pp. 2-5 (Jul. 2002).
Grabski et al., “Automated solubility screening of recombinant proteins in a 96-well format”,Innovations Newsletter, No. 16, pp. 11-13 (Mar. 2003).
Grabski et al., “BugBuster™ and Benzonase®: The clear solutions to simple, efficient extraction ofE. coliproteins”,Innovations Newsletter, No. 10, pp. 17-19.
Grabski et al., “Extraction and purification of proteins fromE. coliwithout harvesting cells”,Innovations Newsletter, No. 13, pp. 1-4 (Nov. 2001).
Loomis et al., “Centrifugation-free protein extraction and purification from total cultures of baculovirus-infected insect cells”,Innovations Newsletter, No. 15, pp. 16-19 (Nov. 2002).
Mehler et al., “Robotic solubility screening and purification of fusion proteins”,Innovations Newsletter, No. 18, pp. 20-22.
Saito et al., “Characteristics of N-octyl β-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry,”Biochem. J., 1984, vol. 22, pp. 829-832.
Bozek Laura L.
Engel Laurie
Johnson Tonny M.
Schultz John W.
Stevens Judith N.
Ford Vanessa L.
McDonnell Boehnen & Hulbert & Berghoff LLP
Promega Corporation
Siew Jeffrey
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