Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2007-11-20
2007-11-20
Landsman, Robert S. (Department: 1647)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S325000, C530S350000, C530S402000
Reexamination Certificate
active
10725103
ABSTRACT:
Cells are engineered to stably or transiently express T1R2 and T1R3 polypeptides, desirably in association with a G protein. The resultant heteromeric taste receptor is a sweet taste receptor and responds to sweet taste stimuli such as artificial and natural sweeteners, e.g., saccharin. The invention provides a preferred mammalian cell lines, e.g., HEK-293 cells that stably express T1R2/T1R3 and Gα15under inducible conditions. These cells are useful in cell-based assays for identifying compounds that elicit or modulate sweet taste.
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Adler Jon Elliot
Echeverri Fernando
Li Xiaodong
O'Connell Shawn
Staszewski Lena
Landsman Robert S.
Senomyx Inc.
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