Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2008-04-29
2008-04-29
Landsman, Robert (Department: 1647)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S325000, C530S350000, C530S402000
Reexamination Certificate
active
07364903
ABSTRACT:
The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.
REFERENCES:
patent: 5993778 (1999-11-01), Firestein et al.
patent: 6383778 (2002-05-01), Zuker et al.
patent: 6540978 (2003-04-01), Margolskee et al.
patent: 2002/0051997 (2002-05-01), Zuker et al.
patent: 2002/0094551 (2002-07-01), Adler et al.
patent: 2002/0151052 (2002-10-01), Chaudhari et al.
patent: 2002/0160424 (2002-10-01), Adler et al.
patent: 2002/0168635 (2002-11-01), Zuker et al.
patent: 2003/0008344 (2003-01-01), Adler et al.
patent: 2003/0022278 (2003-01-01), Zuker et al.
patent: 2003/0022288 (2003-01-01), Zuker et al.
patent: 2003/0036089 (2003-02-01), Wei et al.
patent: 2003/0040045 (2003-02-01), Zuker et al.
patent: 2003/0054448 (2003-03-01), Adler et al.
patent: 2003/0166137 (2003-09-01), Zuker et al.
patent: 2004/0086877 (2004-05-01), Lal et al.
patent: 2005/0106571 (2005-05-01), Erlenbach et al.
patent: 2007/0105159 (2007-05-01), Erlenbach et al.
patent: WO 00/06592 (2000-02-01), None
patent: WO 00/06593 (2000-02-01), None
patent: WO 01/64882 (2001-09-01), None
patent: WO 01/66563 (2001-09-01), None
patent: WO 01/83749 (2001-11-01), None
patent: WO 03/25137 (2002-03-01), None
patent: WO 02/064631 (2002-08-01), None
patent: WO 03/01876 (2003-01-01), None
patent: WO 2005/015158 (2005-02-01), None
Wolfgang Bönigk, et al., “The Native Rat Olfactory Cyclic Nucleotide-Gated Channel is Composed of Three Distinct Subunits”, The Journal of Neuroscience, vol. 19, No. 136, pp. 5332-5347, Jul. 1, 1999.
Jean-Pierre Montmayeur, et al., “A Candidate Taste Receptor Gene Near a Sweet Taste Locus”, Nature Neuroscience, vol. 4, No. 5, May 2001.
Michinori Kitagawa, et al., “Molecular Genetic Identification of a Candidate Receptor Gene for Sweet Taste”, Biochemical and Biophysical Research Communications, vol. 283, pp. 236-242, 2001.
Marianna Max, et al., “Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac”, Nature Genetics, vol. 28, pp. 58-63, May 2001.
Eduardo Sainz, et al., “Identification of a Novel Member of the T1R family of putative taste receptors”, Journal of Neurochemistry, vol. 77, pp. 896-903, 2001.
Claire Johnson, et al., “The Effect of the Sweetness Inhibitor 2(-4-methoxyphenox) propanoic acid (sodium salt) (Na-PMP) on the taste of bitter-sweet stimuli”, Chemical Senses, vol. 19, No. 4, pp. 349-358, 1994.
Sue C. Kinnamon and Thomas A. Cummings, “Chemosensory Transduction Mechanisms in Taste”, Annu. Rev. Physoil., vol. 54, pp. 715-731, 1992.
Xiadong Li, et al., “Human Receptors for Sweet and umami taste”, Proceeding of the National Academy of Science, vol. 99, No. 7, pp. 4692-4696, Apr. 2, 2002.
Database EMBL ′Online!, embl heidelberg; Acc#: Ac062024, Jun. 21, 2000.
E. Adler, et al., “A Novel Family of Mammalian Taste Receptors”, Cell, vol. 100, No. 6, pp. 693-702, Mar. 17, 2000.
Guo, et al., “Protein Tolerance to Random Amino Acid change”, PNAS, vol. 101, No. 25, pp. 9205-9210, Jun. 22, 2004.
Nelson, et al., “Mammalian Sweet Taste Receptors”, Cell, vol. 106, pp. 381-390, Aug. 10, 2001.
Nelson,et al., “An Amino-acid Taste Receptor”, Nature, vol. 416, pp. 199-202, Mar. 14, 2002.
Alexander, et al., “Altering the Antigenicity of proteins”, Proc. Natl. Acad. Sci. USA, vol. 89, pp. 3352-3356, Apr. 1992.
Bowie, et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions”, Science, vol. 247, pp. 1306-1310, Mar. 16, 1990.
Krautwurst, et al., “Identification of Ligands for Olfactory Receptors by Functional Expression of a Receptor Library”, Cell, vol. 95, pp. 917-926, Dec. 23, 1998.
GenBank Accession No. AL139287, clone RP5-89003, Feb. 13, 2000.
GenBank Accession No. AA907022, May 19, 1998.
Hoon, et al., “Putative Mammalian Taste Receptors: A Class of Taste-Specific GPCRs with Distinct Topographic Selectivity”, Cell, vol. 96, pp. 541-551, Feb. 19, 1999.
Lindemann, “A Taste for Umami”, Nature Neuroscience, ol. 3, No. 2, pp. 99-100, Feb. 2000.
Perruccio, et al., “Possible Role for gustducin in Taste Transduction in Hirudo Medicinalis”, Society for Neuroscience Abstracts, vol. 26, No. 1-2, Abstract No. 66.15, 2000.
Adler Jon Elliot
Echeverri Fernando
Li Xiaodong
O'Connell Shawn
Staszewski Lena
Hunton & Williams LLP
Landsman Robert
Senomyx Inc.
LandOfFree
Cell lines that stably or transiently express a functional... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cell lines that stably or transiently express a functional..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell lines that stably or transiently express a functional... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2795200