Cell lines having enhanced cell longevity and protein...

Details Cell lines having enhanced cell longevity and protein... Cell lines having enhanced cell longevity and protein...

2009-09-24

2010-11-30

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S069100, C435S320100, C536S023100

Reexamination Certificate

active

07842493

ABSTRACT:
It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I2-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I2-ank-3 protein compared to expression host cells that are not expressing P-ank-1 and/or I2-ank-3 protein.

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