Cell lines from a transgenic mouse which express biologically ac

Details Cell lines from a transgenic mouse which express biologically ac Cell lines from a transgenic mouse which express biologically ac

1995-02-24

1998-09-29

Crouch, Deborah

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

435 696, 4352401, 435 713, 935 60, C12N 500, C12N 1500, C12P 2104

Patent

active

058147160

ABSTRACT:
Cell lines have been prepared from transgenic mice comprised of a first exogenous DNA sequence encoding human factor IX which is controlled by a promoter region specific for hepatic cells and a second exogenous DNA sequence encoding an oncogene, such as the SV40 virus gene encoding the T-antigen or the mouse c-myc gene, which is controlled by a promoter region specific for hepatic cells. Promoter regions specific for hepatic cells included the promoter region for the factor IX gene and the promoter region for the .alpha.-1 antitrypsin gene. The cultured cells produce biologically active human factor IX which is then isolated from the culture media.

REFERENCES:
K.H. Choo et al, "Expression of Active Human Blood Clotting Factor IX in Transgenic Mice: Use of a cDNA with Complete mRNA Sequence," Nucleic Acids Research, vol. 15, No. 3, 1987 (Oxford, GB) pp. 871-884.
D.S. Anson et al, "The Gene Structure of Human Anti-haemophilic Factor IX," The EMBO Journal, vol. 3, No. 5, 1984, IRL Press Ltd., (Oxford, GB), pp. 1053-1060.
P.H. Reitsma et al, "The Putative Factor IX Gene Promoter in Hemophilia B leyden," Blood, vol. 72, No. 3, Sep. 1988, Grune & Stratton, Inc., (New York, US); pp. 1074-1076 see abstract; p. 1074, col. 2; p. 1075, FIG. 1.
S. Jallet et al, "Characterization of Recombinant Human Factor IX Expressed in Transgenic Mice and in Derived Trans-immortalized Hepatic Cell Lines," Embase Databank, Abstract No. 90288437; see abstract and Embo J. 1990, 9/10 3295-3301.
Choo et al (1987) Nucleic Acids Res. 15, 871-884.
R. Brinster et al 1988) Proced. Natl. Acad Sci, 85, 836-840.
Ciliberto et al (1985) Cell 41, 531-540.
Anson et al (1984) The EMBO J. 3, 1053-1060.
Choo et al (1987) "Expression of Active Human Clotting Factor IX in Transgenic Mice", Nucl. Acids Res. 15, 871-884.
Ciliberto et al (1985) "Cell Specific Expression of a Transfected Human .beta..sub.1 -antitrypsin Gene", Cell 41, 531-540.
Anson et al (1984) "The Gene Structure of Human Anti-Haemophilic Factor IX", EMBO J. 3, 1053-1060.

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