Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1986-04-17
1989-01-17
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435226, 43524021, 435814, C12N 964, C12N 948, C12N 500
Patent
active
047987960
ABSTRACT:
Human cell lines that proliferate in the absence of serum or of any macromolecular growth factors are produced by a process comprising removing serum containing culture medium from the culture of a human cell line that requires serum or macromolecular growth factors for growth, replacing the serum-containing culture medium with culture medium deprived of serum and of any macromolecular growth factors, feeding the adherent or non-adherent cells with serum-free and growth factor-free culture medium using conventional cell culture conditions, culturing the cells until they propagate reproducibly and indefinitely in the absence of serum and of any macromolecular growth factors and a serum and growth factor independent cell line has been established. The novel cell lines can be used for the production of biologically active compounds such as proteins, especially plasminogen activators.
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Kanzaki et al.; "Human Malignant Melanoma In Vivo and In Vitro", J. Nat'l. Cancer Inst., vol. 59 (1977), pp. 775-785.
Bio-Response, Inc.
Weimar Elizabeth C.
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