Cell line producing AIDS viral antigens without producing infect

Details Cell line producing AIDS viral antigens without producing infect Cell line producing AIDS viral antigens without producing infect

1986-04-07

1988-06-21

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

4351721, 435236, 435237, 435239, 435810, 43524025, 436808, 436811, 436813, 935106, 935110, 935 3, 530808, C12Q 170, C12N 1500, C12N 704, C12N 500

Patent

active

047525657

ABSTRACT:
Leu-3.sup.- cells surviving infection with the AIDS retrovirus can be induced with IUdR to express infectious virus. A cellular clone (8E5), isolated by limiting dilution of a mass culture of survivor cells, was found to contain a single, integrated, defective provirus that was consitutively expressed. Although IUdR treatment of 8E5 cells failed to induce infectious virus, cocultivation with Leu-3.sup.+ generated the characteristic syncytia associated with acute AIDS retrovirus infention. The single integrated copy of proviral DNA directs the synthesis of all major viral structural proteins except p64 and p34 as monitored by immunoblotting. Diagnostic reagents and kits in accordance with the present invention are also described.

REFERENCES:
patent: 4520113 (1985-05-01), Gallo et al.
patent: 4659599 (1987-03-01), Gallo et al.
Schupback, J. et al., Science, 224:503-505 (1984).
Allan, J. S. et al., Science, 228:1091-1094 (1985).
Montagnier et al., Science 225:63-66 (1984).
Popovic et al., Science 224:497-500 (1984).
Chermann et al., Science 220:868-871 (1983).
Folks et al., Proc. Natl. Acad. Sci. USA 82:4539-4543 (1985).
Levy et al., Science 225:840-841 (1984).

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