Cell line expressing mutated human tissue-type plasminogen...

Details Cell line expressing mutated human tissue-type plasminogen... Cell line expressing mutated human tissue-type plasminogen...

2001-02-16

2009-12-15

Robinson, Hope A (Department: 1652)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S252300, C435S069100, C435S320100, C435S006120

Reexamination Certificate

active

07632677

ABSTRACT:
The invention involves a cell line which can express human mutated tissue-type plasminogen activator (TNK-TPA), and its preparation methods. The collection number of cell lines in this invention is CCTCC C200006. We first used a DNA fragment from the short arms of human group D or group G chromosomes or its homolog to construct a recombinant human source gene vector-TNK-TPA (collection number is CCTC m200032); TNK-TPA gene is then incorporated into the target site in the nucleolus organizing region (NOR) of human group D or G chromosomes in host cell line HT1080; of which the recombinant cell line is attained after screening. The recombinant cell line can be used for manufacturing protein.

REFERENCES:
patent: 5223422 (1993-06-01), Weidle et al.
patent: 5501853 (1996-03-01), Haigwood et al.
patent: 5656269 (1997-08-01), Haigwood et al.
patent: 7247431 (2007-07-01), Xia et al.
patent: 199574 (1986-10-01), None
patent: 0340573 (1989-11-01), None
patent: 0 838 526 (1998-04-01), None
patent: 1 304 380 (2003-04-01), None
Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990.
Heim et al., PNAS, vol. 91, pp. 12501-12504, 1994.
B.A. Keyt et al., Proc. Natl. Acad. Sci. USA, vol. 91 (Apr. 1994), pp. 3670-3674.

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