Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2005-03-15
2005-03-15
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S006120, C435S091100, C435S091200, C435S091510, C435S287100
Reexamination Certificate
active
06867035
ABSTRACT:
This invention provides a method for selecting a clone of an ES cell containing a mutation in a gene that is expressed in a test cell comprising: (a) providing cDNA obtained by reverse transcription of mRNA of the test cell; (b) providing a collection of cultured ES cells organized into individual clones, wherein each clone is of an ES cell having a mutation in an exon in its genome, the mutation being in a different exon in cells of different clones; (c) providing an array of different single stranded polynucleotides, the polynucleotides being fragments of exons containing mutations in (b); (d) exposing the cDNA to the array under conditions permitting hybridization of polynucleotides in the array to nucleic acids; (e) detecting hybridization of cDNA to a polynucleotide on the array; and, (f) selecting a clone in the collection from which a hybridizing polynucleotide detected at (c) is an exon fragment. This invention also provides a system for testing expression of a gene in a test cell. Also provided is a preferred exon trap vector for mutating ES cells.
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Lu Frank
Speckman Ann W.
The University of British Columbia
Whisenant Ethan
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