Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1982-09-22
1985-04-09
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435240, 435 7, 436533, 436534, 436546, 436548, 436824, 436519, C12N 1500, C12N 500, C12P 2100, G01N 3354
Patent
active
045102449
ABSTRACT:
Method for isolating specific antibody hybridomas from a hybridoma cell mixture employing antigen-conjugated labeled microspheres and a label activated cell sorter. By selecting for labeled cells which produce light scatter and low red autofluorescence, viable single cells can be isolated and cloned which produce the desired antibodies.
REFERENCES:
patent: 4108972 (1978-08-01), Dreyer
patent: 4172124 (1979-10-01), Koprowski et al.
patent: 4196265 (1980-04-01), Koprowski
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4363799 (1982-12-01), Kung et al.
Liesegang et al., P.N.A.S. USA, 75(8):3901-3905 (1978).
Melchers et al., Current Topics in Microbiology and Immunology, 81, Springer-Verlag, New York, IX-XXIII (1978).
Bryan Virginia M.
Herzenberg Leonard A.
Parks David R.
Kepplinger Esther M.
Rowland Bertram I.
The Board of Trustees of the Leland Stanford Jr. University
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