Cell labeling with antigen-coupled microspheres

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 68, 435240, 435 7, 436533, 436534, 436546, 436548, 436824, 436519, C12N 1500, C12N 500, C12P 2100, G01N 3354

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045102449

ABSTRACT:
Method for isolating specific antibody hybridomas from a hybridoma cell mixture employing antigen-conjugated labeled microspheres and a label activated cell sorter. By selecting for labeled cells which produce light scatter and low red autofluorescence, viable single cells can be isolated and cloned which produce the desired antibodies.

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patent: 4363799 (1982-12-01), Kung et al.
Liesegang et al., P.N.A.S. USA, 75(8):3901-3905 (1978).
Melchers et al., Current Topics in Microbiology and Immunology, 81, Springer-Verlag, New York, IX-XXIII (1978).

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