Cell harvester system

Details Cell harvester system Cell harvester system

1989-02-24

1997-07-15

Warden, Jill

Chemistry: molecular biology and microbiology

Apparatus

Means for separation or recovery of a microorganism from...

4352971, 4353051, 422100, 422101, C12M 112, C12M 100

Patent

active

056482663

ABSTRACT:
In a preferred embodiment, there is provided a cell harvester system, and method for operating the same, which furnishes a flow of wash solution into each of, for example, the 96 wells of a microplate. The contents of each well are aspirated to a specific area on a filter mat and the effluent is directed to one of two traps. Separate areas of the filter mat unique to each well are isolated by means of opposing O-rings. Due to the proximity of the sample wells (9 mm on center) there is the possibility of an overflow from one well contaminating adjacent wells. To prevent that occurrence, an aspirated overflow ring space is provided around each well. A microprocessor controlled system is used to sequence the various functions. To provide flexibility of control, the 96-well plate is treated as 8 separate 12-well systems (rows). Each row of 12 wells has its own overflow valve, wash valve, and aspirate valve. The control system can sequence any number or rows at a time and the control system can be set to select from one of a plurality of wash solutions.

REFERENCES:
patent: 3568735 (1971-03-01), Lancaster
patent: 4201845 (1980-05-01), Feder et al.
patent: 4245042 (1981-01-01), Weinstein et al.
patent: 4383041 (1983-05-01), Kutsusawa et al.
patent: 4493896 (1985-01-01), La Motte, III et al.
Brochure on Skatron cell harvester and filterdisc transfer system by Skatron Inc., Sterling, Virginia (No Date).
Brochure on Brandel cell harvesters by Brandel, Gaithersburg, Maryland (No Date).
Brochure on PHD cell harvester by Cambridge Technology, Inc., Cambridge, Massachusetts (No Date).
Brochure on Skatron automatic microplate washers by Skatron Inc., Sterling VA (No Date).
Brochure on automated microplate washer by Bio-Tek Instruments, Inc., Burlington VT (No Date).
Brochure on microplate washer by SLT Labinstruments, Salzburg, Austria (No Date).
Brochure on automated wash system by Perkin-Elmer, Norwalk CT.

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