Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1982-11-19
1985-04-30
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435948, 435240, C12N 502, C12N 500, C12R 191
Patent
active
045145008
ABSTRACT:
Droplets having an electrical charge on the surface thereof are prepared from a first liquid and dispersed in a second liquid, a sterile aqueous tissue culture medium containing serum proteins. The first liquid is non-toxic to living cells and is relatively immiscible with water. The electrical charge on the surfaces of the droplets has a charge density in the range of from about 10 to about 100 charges per square nanometer. Serum protein films adhere to the charged droplets strongly enough to prevent rupture by human fibroblast cells grown thereon.
REFERENCES:
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W. L. McKeehan, et al., "Stimulation of Clonal Growth of Normal Fibroblasts with Substrata Coated with Basic Polymers", [The Journal of Cell Biology, vol. 71, (1976), pp. 727-734].
P. F. Davies, "Microcarrier Culture of Vascular Endothelial Cells on Solid Plastic Beads", [Experimental Cell Research 134, (1981), pp. 367-376].
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"Cultivation of Living Cells on Liquid Perfluorocarbons", by Ivanitskii et al.; translated from Doklady Akademii Nauk SSSR, vol. 258, No. 1, pp. 225-228, May, 1981.
Giaever Ivar
Keese Richard C.
Davis Jr. James C.
General Electric Company
Magee Jr. James
MaLossi Leo I.
Tarcza John E.
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