Cell growth medium supplement

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435244, 435241, 435 2, 424101, 260112B, C12N 500, C12N 502, A61K 3516, A01N 100

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active

044528930

ABSTRACT:
Fraction IV, a discard fraction of the Cohn fractionation scheme, may be used as a supplement in cell growth media if the Fraction IV is rendered substantially free of components within the molecular weight range 2.5.times.10.sup.5 -1.0.times.10.sup.10, which components inhibit cell growth.

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patent: 4198479 (1980-04-01), Tytell et al.
patent: 4216205 (1980-08-01), Radowitz
patent: 4251510 (1981-02-01), Tankersley
Miles Research Products 1976, Miles Laboratories, Inc., Elkhart, IN 46514.
Cohn et al., "Preparation and Properties of Serum and Plasma Proteins," Journal of the American Chemical Society: vol. 68 (1946), pp. 459-475.
Ham et al., "Media and Growth Requirements," Methods in Enzymology, vol. 58 (1979), p. 92.
Maciag et al., "An Endocrine Approach to the Control of Epidermal Growth: Serum-Free Cultivation of Human Keratinocytes," Science vol. 211 (3-1981), pp. 1452-1454.
Rinderknecht et al., "Polypeptides with Nonsuppressible Insulin-like and Cell-Growth Promoting Activities in Human Serum," Proceedings of the National Academy of Sciences, vol. 73(7), (1976), pp. 2365-2369.

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