Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Reexamination Certificate
1999-08-27
2001-12-25
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
C435S325000, C435S404000, C424S601000
Reexamination Certificate
active
06333193
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to a cell growth accelerator and a cell growth method using the same. More specifically, it relates to a cell growth method using a polyphosphoric acid as a cell growth accelerator.
DESCRIPTION OF THE RELATED ART
When animal cells are cultivated in vitro, a medium obtained by adding serum as a cell growth factor in an amount of from 10 to 20% to a basal medium containing amino acids, vitamins, saccharides and inorganic salts has been generally used. However, the serum cannot be mass-produced and is therefore quite expensive, and further, each individual composition (by lot) differs greatly. Since the amount of one lot is limited, intricate procedures such as lot checking, preparation and control of culture conditions are required whenever the lot changes.
In many cases, when substances (particularly, proteins including enzymes, physiologically active substances, vaccines and viruses) are produced using culture cells, serum is required. The development of a technique in which to minimize the amount of serum used or to use a serum-free medium has been underway. Instead of serum, various cell growth factors or hormones, such as pituitary extract, fibroblast growth factors and insulin, are added to a basal medium to expedite the cell growth. However, since growth factors or hormones added instead of serum are expensive, the cost for maintaining culture cells becomes high. Further, there is a likelihood that pathogens such as viruses and prions are incorporated in serum, and contamination with such pathogens is an issue.
Further, various medications have been used for treatment of injuries, burns or wounds in patients undergoing a surgical operation. However, there is no medication to expedite the tissue repair by accelerating the cell growth. Treatment is indirectly expedited by preventing bacterial infection with, for example, antibiotics. In the treatment of burns, an artificial skin effective for expediting regeneration of tissue and accelerating healing has been put to practical use. However, a substance that actively functions, as an ingredient of artificial skin, to expedite the cell growth and accelerate healing has been relatively unknown, except for natural proteins such as collagen. Moreover, in treatment of alveolar pyorrhea, a periodontal disease, material comprising of natural substances, including proteins such as a periodontium regeneration factor, has been under development. Nevertheless, it has not yet been put to practical use.
Furthermore, with respect to the problem of hair growth promotion, various extracts of natural substances, synthetic agents or estrogens, having the functions of hair matrix cell activation and blood flow promotion, have been reported as effective components, and pharmaceuticals or medicated toiletries for hair growth promotion have been provided. For example, a hair growth promotor obtained by mixing a scalp activation promotor (such as camphor, capric acid, phenol or salicylic acid) with an extract of
Houttuynia cordata, Artemisia indica
or an aloe is described in JP-A-10-194935. Further, a hair growth promotor containing an extract of
Geranium nepalense
ssp.
thunbergii
as a cell activator is described in JP-A-9-227342. Still further, a product obtained by mixing a blood flow promotor with naphthalenesulfonic acid and/or benzophenonesulfonic acid is described in JP-A-8-40835, and a hair growth promotor containing L-menthol, sodium p-toluenesulfonyl chloroamide, sodium hypochlorite or D-pantothenyl alcohol is described in JP-A-7-53334. Thus, a large number of hair growth promoters have been so far introduced.
However, the effect of hair growth promotion using these hair growth promoters has not necessarily been satisfactory.
SUMMARY OF THE INVENTION
It is an object of the invention to accelerate cell growth in cell cultures safely and efficiently with low cost, using a medium and a cell growth material in which cell growth can be accelerated, when produced in vitro with culture cells.
That is, the invention relates to a cell growth accelerator containing a linear condensed polyphosphoric acid and/or a polyphosphate.
Further, the invention relates to a cell growth accelerator wherein the cells are hair matrix cells.
Furthermore, the invention relates to a cell growth accelerator wherein the cells are bone cells.
Further, the invention relates to a cell growth method wherein a polyphosphoric acid is added to a medium for cells of animals and plants.
Still further, the invention relates to a cell growth method, wherein polyphosphoric acid is added to a medium for cells of animals and plants containing from 0 to 10% serum.
Furthermore, the invention relates to a cell growth method, wherein polyphosphoric acid is added to a medium for cells of animals and plants containing a cell growth factor or/and various physiologically active factors.
Moreover, the invention relates to a cell growth method, wherein the cells are grown in a cell cultivator containing, or coated with, polyphosphoric acid.
REFERENCES:
patent: 4115199 (1978-09-01), Porubcan et al.
patent: 4615977 (1986-10-01), Hasegawa et al.
patent: 5108755 (1992-04-01), Daniels et al.
Kubovcik & Kubovcik
Lankford , Jr. Leon B.
Nipro Corporation
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