Cell fusion apparatus

Details Cell fusion apparatus Cell fusion apparatus

1989-03-13

1990-09-25

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

435290, 935 93, 363 85, 2041801, 204299R, C12M 138, C12M 300, B01D 6142

Patent

active

049593218

ABSTRACT:
Cell electrofusion apparatus, for example for the production of hybridomas, comprises a flow-through capillary (14) surrounded by a water-cooled jacket (16) and through which a physiologically normal medium (such as a tissue culture fluid) containing cells is pulsed by a pump driven syringe (28), high voltage pulses being applied to electrodes (18) at opposite ends of the channel, which voltage pulses are of a magnitude and duration such that, taking into account the rate of fluid flow through the capillary and the extraction of heat by the cooling means, the temperature of the cell-containing medium is constrained to remain below the limit at which substantial cell damage occurs.

REFERENCES:
patent: 4476004 (1984-10-01), Pohl
patent: 4578168 (1986-03-01), Hofmann
patent: 4622302 (1986-11-01), Sowers
patent: 4695547 (1987-09-01), Hilliard et al.
patent: 4750100 (1988-06-01), Ragsdale
patent: 4764473 (1988-08-01), Matschke
patent: 4800163 (1989-01-01), Hibi et al.
patent: 4804450 (1989-02-01), Mochizuki et al.
patent: 4832814 (1989-05-01), Root
U. Zimmermann et al., "Electric Field-Induced Cell-to-Cell Fusion", Journal of Membrane Biology, vol. 69, 1982, pp. 165-182.

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