Cell fusion

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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4351722, 435240, 435948, 424 85, 935 93, 935100, 935106, C12P 2100, C12N 1500, C12N 500, C12R 1191

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045296944

ABSTRACT:
Human hybridomas producing a preselected human monoclonal antibody are prepared by fusing human lymphocytes with a hybrid fusion partner. The hybrid fusion partner is the result of fusing human lymphocytes with human myeloma cells at least once wherein the resulting hybrid cell is capable of being a functional human fusion partner.

REFERENCES:
Olsson et al, "Human Human Hybridomas Producing Monoclonal Antibodies of Predefined Antigenic Specificity", Proceedings of the National Academy of Sciences, 77(9), (1980), pp. 5429-5431.
Shulman et al, "A Better Cell Line for Making Hybridomas Secreting Specific Antibodies", Nature, 276, (11-1978), pp. 269-270.
Weiss, "Cell Hybridization: A Tool for the Study of Cell Differentiation", Somatic Cell Genetics by Caskey et al, (1982), pp. 169-182.
Levy et al, "Mouse-Human Hybridomas. The Conversion of Non-Secreting Human B Cells into Ig Secretors", Current Topics in Microbiology and Immunology, 81, (1978), pp. 170-172.
Galfre et al, "Chemical Typing of Human Kappa Light Chain Subtypes Expressed by Human Hybrid Myelomas", Immunology, 45, (1982), pp. 125-128.
Laskov et al, "Induction of IgM Secretion by Fusing Murine B-Lymphoma with Myeloma Cells", Current Topics in Microbiology and Immunology, 81, (1978), pp. 173-175.
Kamei et al., (1968), Experientia, 24, 410.
Krueger et al., (1974), J. Immunol., 112, 1415.
Steinitz et al., Nature, (London, 1977), 269, 420.
Schwaber et al., Nature, (London, 1973), 244, 444.
Kohler et al., Nature, (London, 1975), 256, 495.
Croce et al., Nature, (London, 1980), 288, 488.

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