Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1982-09-28
1986-03-25
Metz, Andrew H.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 2728
Patent
active
045781672
ABSTRACT:
Apparatus for fusing cells by applying an alternating voltage across a pair of spaced-apart elongated electrodes. The electrodes form between themselves an elongated fusion chamber for receiving a suspension of cells to be fused. The electrodes have a cross-sectional shape adapted to produce an inhomogenous electric field within the fusion chamber suitable for inducing dielectrophoretic movement of suspended cells towards the electrodes and for maintaining strings or lines of two or more cells in contact with the electrodes along an elongated, narrow attachment zone on each electrode. A voltage pulse is applied across the electrodes after the cells have become attached thereto. The duration and amplitude of the pulse is selected to be sufficient to cause the strings of cells to fuse into a single cell. The depth of the fusion chamber below the level of the attachment zones is less than 2 mils, which is shallow enough to inhibit collection of cells below the attachment zones, thereby improving fusion efficiency.
REFERENCES:
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Biofusion, Inc.
Boggs Jr. B. J.
Metz Andrew H.
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