Cell-free synthesis and isolation of novel genes and polypeptide

Details Cell-free synthesis and isolation of novel genes and polypeptide Cell-free synthesis and isolation of novel genes and polypeptide

1991-11-29

1997-08-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 681, 435 692, 435 693, 435 694, 435 695, 435 696, 4351723, 4351721, 435 3, 435 4, 435 10, 435 17, C12P 2102, C12N 1500

Patent

active

056587546

ABSTRACT:
A method for the cell-free synthesis and isolation of novel genes and polypeptides is provided. Within one embodiment, an expression unit is constructed onto which semi-random nucleotide sequences are attached. The semi-random nucleotide sequences are first transcribed to produce RNA, and then translated under conditions such that polysomes are produced. Polysomes which bind to a substance of interest are then isolated and disrupted; and the released mRNA is recovered. The mRNA is used to construct cDNA which is expressed to produce novel polypeptides.

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