Cell-free synthesis and isolation of novel genes and polypeptide

Details Cell-free synthesis and isolation of novel genes and polypeptide Cell-free synthesis and isolation of novel genes and polypeptide

1995-06-06

1997-07-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9151, 435611, 435 601, 935 16, 935 17, 935 19, 935 20, 935 21, 935 3, 935 4, 935 10, 536 231, C12P 1934, C12N 1511

Patent

active

056437684

ABSTRACT:
A method for the cell-free synthesis and isolation of novel genes and polypeptides is provided. Within one embodiment, an expression unit is constructed onto which semi-random nucleotide sequences are attached. The semi-random nucleotide sequences are first transcribed to produce RNA, and then translated under conditions such that polysomes are produced. Polysomes which bind to a substance of interest are then isolated and disrupted; and the released mRNA is recovered. The mRNA is used to construct cDNA which is expressed to produce novel polypeptides.

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