Cell-free receptor binding assays, the production and use thereo

Details Cell-free receptor binding assays, the production and use thereo Cell-free receptor binding assays, the production and use thereo

1994-05-13

1997-06-17

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 72, 435 75, 435 78, 435 792, 435 28, 436518, C12Q 170, C12Q 128, C12Q 142, C12Q 166, G01N 33543

Patent

active

056395973

ABSTRACT:
The invention relates to cell-free receptor binding assays which permit the binding behavior of receptor proteins in the cell membrane toward natural or artificial ligands to be investigated. This entails the particular receptor being linked to a suitable carrier molecule, preferably the heavy chain of an immunoglobulin, and being bound via the carrier, with retention of its biological property, to a suitable solid phase.

REFERENCES:
patent: 4859609 (1989-08-01), Dull et al.
patent: 5116964 (1992-05-01), Capon et al.
Sankolli et al., Improvement in the antibody binding characteristics of microtitre wells by pretreatment with anti-IgG Fc immunoglobulin. Journal of Immunological Methods 104:191-194, 1987.
Watson et al., J. Cell Biol 110:2221-2229, 1990.
Ashkenagi et al., Proc. Natl. Acad. Sci. 87:7150-7154, 1990.
Radioiodination of Interleukin 2 to High Specific Activities by the Vapor-Phase Chloramine T Method, by John J. Siekierka and Steven DeGudicibus, Analyt. Biochem. 172:514-517, Feb. 25, 1988.
Nonradioactive Ligand Binding Assay for Epidermal Growth Factor by Ivan C. King and Joseph J. Catino, Analyt. Biochem. 188:97-100, Nov. 5, 1990.
"Visualization of Binding Sites for Bovine Parathyroid Hormone (PTH 1-84) on Cultured Kidney Cells With a Biotinyl-b-PTH(1-84) Antagonist 1,2", by A. Niendork, et al., J. Histochem. Cytochem. 34(3): 357-361, 1986.
"A Receptor for Tumor Necrosis Factor Defines an Unusual Family of Cellular and Viral Proteins", by Craig A. Smith, et al., Science 248: 1019-1023, 1990.
"Expression Cloning of a Receptor for Human Granulocyte-Macrophage Colony-Stimulating Factor" by David P. Gearing, et al., Embo J. 8(12): 3667-3676, 1989.
"The For Receptor of Natural Killer Cells is a Phospholipid Linked Membrane Protein", by David Simmons, et al., Nature 333: 568-570, 1988.
"Molecular Cloning of a CD28 cDNA by a High-Efficiency COS cell Expression System", vol. 84, by Alejandro Aruffo, et al., Proc. Natl. Acad. Sci. 84:8573-8577, 1987.
"An LFA-3 cDNA encodes a Phospholipid-linked Membrane Protein Homologous to Its Receptor CD2" by Brian Seed, Nature 329 (29):840-842, 1987.

Affiliated with

Also associated with

Rating

Cell-free receptor binding assays, the production and use thereo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell-free receptor binding assays, the production and use thereo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell-free receptor binding assays, the production and use thereo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2156910