Cell-free protein synthesis system with protein translocation an

Details Cell-free protein synthesis system with protein translocation an Cell-free protein synthesis system with protein translocation an

1998-03-20

2000-08-15

Slobodyansky, Elizabeth

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435325, C12P 2100, C12N 506

Patent

active

061034894

ABSTRACT:
An amphibian egg extract is added to a cell-free translation system to obtain in vitro translocation and processing of newly synthesized peptide. The egg extract produces near complete signal sequence and N-linked carbohydrate processing of protein. Egg extract microsomes provide for protein translocation.

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Laemmli, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4," Nature 227:680-685 (1970).
Matthews and Colman, "A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs," Nucl. Acids Res., 19:6405-6412 (1991).
Murray and Kirschner, "Cyclin synthesis drives the early embryonic cell cycle," Nature 339:275-280 (1989).
Zhou et al., "In vitro translation of the human insulin proreceptor results in N-linked glycosylation without dimer formation," Biochem. Biophys. Res. Comm., 192:1453-1459 (1993).
Tsuda et al., "The in vitro synthesized and processed human insulin receptor precursor binds insulin," FEBS Lett., 457:13-17 (1999).
Zhou et al. (Dec. 1996) Mol. Biol. of the Cell, vol. 7, p. 439a, abstract 2552.

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