Cell-free extract and glycoprotein synthesis system

Details Cell-free extract and glycoprotein synthesis system Cell-free extract and glycoprotein synthesis system

2002-11-25

2011-10-11

Burkhart, Michael (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

Reexamination Certificate

active

08034581

ABSTRACT:
Insect cells are stored in a small gas cylinder, and the small gas cylinder is charged with nitrogen gas to pressurize the cylinder. The charged gas is exhausted at once to crush the cells to provide the objective cell extract with translation activity and glycosylation activity. As this method is gentler than the conventional cell-crushing method employing a homogenizer, in addition to translation factors, factors carrying glycosylation activity can also be recovered. As a result, an in-vitro glycoprotein synthesis system capable of performing translation to post-translation glycosylation can be produced.

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