Cell-free and whole cell ice nucleators and process for their pr

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

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435 691, 4351723, 43525233, 43525234, 435847, 435874, 530324, 530828, 252 70, C12R 2104, C12R 118, C12R 138, C12N 1500

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052234126

ABSTRACT:
Microbially produced ice nucleator mixtures which include either cell-free ice nucleator particle mixtures and/or whole cell ice nucleator mixtures. These mixtures are produced in methods which comprises culturing a selected microorganism in a two step process at a first temperature in a first step and at a lower temperature in a second step. The mciroorganisms include Erwinia, Pseudomonas and Escherichia coil. These methods produce ice nucleator mixtures having increased concentrations of ice nucleating sites per given weight or volume of ice nucleator material.

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