Cell fixative composition and method of staining cells without d

Details Cell fixative composition and method of staining cells without d Cell fixative composition and method of staining cells without d

1992-03-27

1995-06-06

Russel, Jeffrey E.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436 18, 436174, 436805, 435 29, 435 405, G01N 3100, G01N 100, C12Q 102

Patent

active

054222770

ABSTRACT:
A cell fixative composition for fixing the internal components of a cell without disrupting the cell surface components is disclosed. The composition contains a compound for preserving intracellular morphology, a compound for fixing internal components of the cell, a compound which facilitates transportation of components across the cellular membrane, and a compound for increasing permeability of cell membranes. Methods for analyzing cells which have been fixed using the composition are also disclosed.

REFERENCES:
patent: 4423153 (1983-12-01), Ranney
patent: 4499183 (1985-02-01), Sujansky et al.
patent: 4632907 (1986-12-01), Sato
patent: 4698312 (1987-10-01), Wong
patent: 4933293 (1990-06-01), Kuroda et al.
H. Sumner et al., Journal of Immunological Methods, 136(1991), pp. 259-267.
L. Zamboni et al. the Journal of Cell Biology, vol. 35, 1967, p. 148A.
The Chemistry and Practice of Fixation, pp.97-158, 1979.

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