Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Reexamination Certificate
1998-10-02
2001-02-27
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
C435S040510, C435S007210, C435S975000
Reexamination Certificate
active
06194165
ABSTRACT:
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
Not Applicable
BACKGROUND
This invention relates to compositions which are used to prepare cell samples for cytological examination, especially where the samples are urine or bladder wash samples or fine needle aspirations of prostate or kidney tissues or organs.
Cytologic analyses are often carried out in central laboratories. Preparation and preservation of the cells and minimization of artifacts is a crucial consideration in shipping samples to central laboratories. With urine samples, among the more common interfering artifacts are crystals that form from the oxalates and phosphates within the urine. Although other substances in the urine can also form crystals, by far the most common are phosphates and oxalates. The crystals can sometimes be so dense as to obscure the cells, interfere with proper staining of the cells, interfere with adherence of the cells to microscope slides, or complicate the analysis of the cells.
In spite of the problems caused by crystal formation within urine and bladder wash samples, there are no cell fixative or preservative compositions for fixing urine which are effective in inhibiting the formation of crystals.
A cell preparative composition which preserved the cells with the retention of the characteristic cell morphology and which simultaneously inhibited crystal formation in the sample or dissolved crystals already within the sample and which also promoted adherence of cells to examination slides would be of great value in the field of cytological analyses.
REFERENCES:
patent: 3975156 (1976-08-01), Kraft et al.
patent: 4534881 (1985-08-01), Sikes et al.
patent: 4992365 (1991-02-01), Hyman
“Fix & Perm—Applications Guide to Intracellular Flow Cytometry” CALTAG Catalog, CALTAG Laboratories, Inc. Burlingame, CA, Aug. 1996.
W.L. Parry et al., “Cancer Detection By Quantitative Fluorescence Image Analysis”,The Journal of Urology, vol. 139, Feb. 1988, pp. 270-274.
P. L. Jones et al., Quantitative Immunofluorescence, Anti-ras p21 Antibody Specificity, and Cellular Oncoprotein Levels,Biochemical and Biophysical Research Communications, vol. 167, No. 2, 1990, pp. 464-470.
J.Y. Rao et al., “Cellular F-Actin Levels as a Marker for Cellular Transformation: Relationship to Cell Division and Differentiation”,Cancer Research, 50, Apr. 1990, pp. 2215-2220.
Rodriguez et al.,Recombinant DNA Techniques: An Introduction, The Benjamin/Cummings Publishing Company, Inc., Menlo Park California, pp. 200-201, 1983.
Kagedal et al., “Automated High-Performance Liquid Chromatographic Determination of 5-s Cysteinyl-3 4-Dihydroxyphenylalanine in Urine,”J Chromatogr, 473(2), Abstract 7175415.
Bonner Rebecca B.
Hemstreet, III George P.
Hurst Robert E.
Dunlap Codding & Rogers P.C.
Le Long V.
Nguyen Bao-Thuy L.
The Board of Regents of the University of Oklahoma
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