Cell enumeration immunoassay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 795, 435967, 435974, 424534, 4241541, 436524, 436531, 436541, 436546, 436548, 436 63, 436172, 436811, C01N 33566

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056587457

ABSTRACT:
This invention relates to a cell enumeration immunoassay which uses a calibrated standard, i.e., a substance which behaves like the sample under study and the concentration of which can be correlated to the concentration of the cells. This immunoassay is an efficient alternative to flow cytometry.

REFERENCES:
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patent: 4677061 (1987-06-01), Rose et al.
patent: 5385822 (1995-01-01), Melnicoff et al.
Franke et al., Quantitative Determination of CD4/CD8 Molecules by a Cell Marker ELISA, Clin. Chem., 40/1, pp. 38-42, 1994.
Carriere et al., An Immunoassay of CD4 and CD8 Antigens on the Surface of Lymphocytes of Healthy Volunteers and HIV Patients, Int-Conf-AIDS, 7(1), p. 259, Jun. 16-21, 1991.
Baumgarten, Horst, A cell ELISA for the quantitation of leukocyte antigens, Journal of Immunological Methods, 94, pp. 91-98, 1986.
Hessian et al., Development of an enzyme immunoassay for the quantitation of cellular antigen expression, Journal of Immunological Methods, 94, pp. 29-34, 1986.

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