Cell-driven viral transfer in eukaryotes

Details Cell-driven viral transfer in eukaryotes Cell-driven viral transfer in eukaryotes

1982-04-05

1984-08-07

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351721, 4351722, 435240, 435948, 424 85, 935 93, 935 71, 935100, 935 89, C12P 2100, C12N 1500, C12N 500, C12R 191

Patent

active

044644655

ABSTRACT:
Novel method, cells and compositions are provided involving transforming B-lymphocytes to provide immortalization for continuous production of monoclonal antibodies to a predetermined ligand. T-cell free B-lymphocytes are combined with an Epstein-Barr virus transformed cell sensitive to a cytotoxic agent which does not significantly affect the B-lymphocytes under conditions where the sensitive EBV transformed cell acting as the transfer agent is killed and efficiently transforms the B-lymphocyte recipient cells with EBV. The EBV transformed B-lymphocyte cells are amplified and cloned, the desired clones isolated in accordance with conventional techniques and then used for production of monoclonal antibodies.

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