Chemistry: molecular biology and microbiology – Apparatus – Involving lysis of a microorganism by means other than...
Patent
1994-03-14
1995-11-07
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Involving lysis of a microorganism by means other than...
435259, 4352831, 241 2, 241175, 366209, C12M 133
Patent
active
054647738
ABSTRACT:
An improved apparatus for effectively disrupting biological samples contained in cuvettes to which beads have been added. In the apparatus, a special arm/bearing subassembly is driven and oscillated by a motor in a manner to attain cellular disruption of the biological samples without degradation of their cellular components. In the preferred form, the special arm/bearing subassembly has a cam, bearings, and a bearing sleeve which cooperate with a motor drive shaft to rotate a yoke with two arms holding four cuvettes. For increased safety and environmental protection, special sample retainers can be provided to better secure the cuvettes and the arm/bearing subassembly is enclosed in a sample chamber which provides a secondary containment compartment that contain any spillage.
REFERENCES:
patent: 3286739 (1966-11-01), Ikakura
patent: 3286939 (1966-11-01), Karpenko et al.
patent: 4125335 (1978-11-01), Blume et al.
patent: 4202634 (1980-05-01), Kraft et al.
patent: 4295613 (1981-10-01), Moore et al.
patent: 4829230 (1989-05-01), Perry et al.
patent: 4874137 (1989-10-01), Chiba
Foster. "Cell Disruption: Breaking Up is Hard to Do". Biotechnology vol. 10 (1992) pp. 1539-1541.
F. O. Schmitt, C. H. Johnson and A. R. Olson. Oxidation Promoted By Ultrasonic Radiation. Chemical Laboratory of Univ. of Cal. Feb. 1929, vol. 51, pp. 370-375.
E. S. Maculla and P. B. Cowles. The Use Of Glycine in the Disruption of Bacterial Cells. Science, Apr. 9, 1948, vol. 107, pp. 376-377.
H. K. King and H. Alexander. The Mechnical Destruction of Bacteria Univ. of Edinburgh, 1948, pp. 315-324.
H. Mickle. Il.-Tissue Disintegrator. Journ. R. MICR. SOC., 1948, pp. 10-12.
P. M. Nossal, A Mechanical Cell Disintegrator. Biochemistry Dept., Univ. of Adelaide, Austral, 1953; 31. pp. 583-590.
R. Davies. Observations On The Use Of Ultrasound Waves For The Disruption of Micro-Organisms. Biochemistry Dept., Univ. of Cambridge, (Great Britain) Aug. 6, 1958, vol. 33, pp. 481-493.
P. Doty, B. Bunce McGill, and S. A. Rice. The Properties Of Sonic Fragments Of Deoxyribose Nucleic. Chemistry Dept. Harvard Univ. Cambridge, Mass., Mar. 25, 1958, vol. 44, pp. 432-438.
C. Lamanna and M. F. Mallette. Use Of Glass Beads for The Mechanical Rupture Of Microorganisms In Concentrated Suspensions. Microbiology and Biochemistry Dept., Johns Hopkins Univ. School of Hygiene and Public Health, Baltimore, Md., Nov. 16, 1953, pp. 503-504.
B. S. Roberson and J. H. Schwab. Studies On Preparation Of Bacterial Cell Walls And Criteria Of Homogeneity. Biochimica et Biophysica ACTA 44, Univ. of N.C School of Medicine, Apr. 12, 1960, pp. 436-444.
A. Rodgers and D. E. Hughes. The Disintegration Of Micro-organisms By Shaking With Glass Beads. Journ. of Biochemical and Microbiological Tech. and Eng. vol. II, No. 1. 1960, pp. 40-70.
W. R. Johnson and C. C. Rice. Shear Degradation Of Vinly Polymers In Dilute Solution By High-Speed Stirring. Journal of Polymer Science, vol. XIV, 1960, pp. 217-225.
D. E. Hughes. The Disintegration Of Bacteria And Other Microorganisms By the M.S.E.-Mullard Ultrasonic Disintegrator. Journal of Biochemical and Microbiological Tech. and Eng., vol. III, No. 4, 1961, pp. 405-433.
F. D. Rumscheidt and S. G. Mason. Particle Motions In Sheared Suspensions. Journal of Colloid Science 16, 1961, pp. 238-261.
P. B. Hamilton and S. G. Knight. Ballistic Disruption of Penicillium Chrysogenum Cells. Dept. of Bacteriology, Univ. Wis. Madison, Jun. 15, 1962, pp. 577-579.
D. E. Hughes and W. L. Nyborg. Cell Disruption by Ultrasound. Science vol. 138., Oct. 12, 1962, pp. 108-114.
S. A. Hawley, R. M. Macleod, and F. Dunn. Degradation Of DNA By Intense, Noncavitating Ultrasound. The Journal Of The Acoustical Society Of America, vol. 35, No. 8, Aug. 1963, pp. 1285-1287.
O. C. Richards and P. D. Boyer. Chemical Mechnanism Of Sonic, Acid, Alkaline And Enzymic Degradation Of DNA. J. Mol. Bio. (1965)II, pp. 327-340.
E. A. Neppiras and D. E. Hughes. Some Experiments On The Disintegration of Yeast By High Intensity Ultrasound. Biotechnology and Bioengineering, vol. VI., 1964, pp. 247-270.
J. W. T. Wimpenny Ph.D. Breakage Of Micro-Organisms. Process Biochemistry, Jul. 1967, pp. 41-44.
A. R. Peacocke and N. J. Prichard. The Ultrasonic Degradation Of Biological Macromolecules Under Conditions Of Stable Cavitation. II. Degradation Of Deoxyribonucleic Acid. Biopolymer, vol. 6, 1968, pp. 605-623.
J. Rehacek, K. Beran and V. Bicik. Disintegration Of Microorganisms And Preparation Of Yeast Cell Walls In A New Type Of Disintegrator. Applied Microbiology, Mar. 1969, pp. 462-466.
E. Work. Cell Walls Mehtods In Microbiology, vol. 5A, 1971, pp. 361-418.P.
D. E. Hughes, J. W. T. Wimpenny and D. Lloyd. The Disintegration Of Micro-Organisms. Norris, Ribbons 1971, Microbiology Department, Univ. College, Cardiff, Glam., pp. 1-53.
C. J. James, W. T. Coakley and D. E. Hughes, Kinetics Of Protein Release From Yeast Sonicated In Batch And Flow Systems At 20kHz. Biotechnology and Bioengineering, vol. XIV, 1972, pp. 33-42.
J. A. Rooney. Shear As A Mechanism For Sonically Induced biological Effects. The Journal Of the Acoustical Society Of America, vol. 52, No. 6 (Part 2) May 12, 1972, pp. 1718-1724.
J. A. Currie, P. Dunnill and M. D. Lilly. Release Of Protein From Bakers' Yeast (Saccharomyces Cerevisiae) By Disruption In An Industrial Agitator Mill. Biotechnology and Bioengineering, vol. XIV, (1972), pp. 725-736.
F. Marffy and M. R. Kula. Enzyme Yields From Cells Of Brewer's Yeast Disrupted By Treatment In A Horizontal Disintegrator. Biotechnology and Bioengineering, vol. XVI, (1974), pp. 623, 634.
A. Helenius and K. Simons. Solubilization Of Membranes by Detergents. Biochimica et Biophysica Acta, 415 (1975) pp. 29-79.
W. T. Coakley, A. J. Bater and D. Lloyd. Disruption Of Micro-Organisms. Adv. Microlo. Physiol. 16 (1977) pp. 279-341.
W. B. Clark, L. L. Bammann and R. J. Gibbons. Comparative Estimates Of Bacterial Affinities And Adsorption Sites On Hydroxyapatite Surfaces. American Society For Microbiology, vol. 19, No. 3, pp. 846-853 (1978).
R. W. H. Verwer, E. H. Beachey, W. Keck, A. M. Stoub and J. E. Poldermans. Oriented Fragmentation Of Escherichia Coli Sacculi By Sonication. Journal Of Bacteriology, Jan. 1980, pp. 327-332.
C. A. Schnaitman. Cell Fractionation. Gerhardt, p. ed., 1981, pp. 52-61.
A. S. Fischel and G. M. Carman. Phosphatidylinositol Biosynthesis In Saccharomyces Cerevisiae: Purification and Properties of Microsome Associated Phosphatidylinositol Synthase. Journal of Bacteriology, Apr. 1983, pp. 304-311.
D. A. Dooley, P. G. Sacks and Morton W. Miller. Production Of Thymine Base Damage In Ultrasound-Exposed EMT6 Mouse Mammary Sarcoma Cells. Radiation Research 97, 1984, pp. 71-86.
T. Kondo, S. Arai, M. Kuwabara, G. Yoshii and E. Kano. Damage In DNA Irradiated With 1.2 MHz Ultrasound and Its Effects On Template Activity Of DNA For RNA Synthesis. Radiation Research 104, 1985, pp. 284-292.
A. F. Kalb, J. C. Loper, C. R. Dey, C. W. Woods and T. R. Sutter. Isolation Of A Cytochrome P-450 Structural Gene From Saccharomyces Cerevisiae. Dept. of Microbiology and Molecular Genetics, and Department of Environmental Health, Univ. of Cincinnati College of Medicine, Cincinnati, Ohio, 1986, pp. 237-245.
M. R. Kula and H. Schutte. Purification Of Proteins And The Disruption Of Microbial Cells. Biotechnology Progress (vol. 3 No. 1) Mar., 1987, pp. 31-42.
H. Schutte and M. R. Kula. Analytical Disruption Of Microorganisms In A Mixer Mill. Enzyme Microb. Technol., 1988, vol. 10, pp. 552-558.
U. Bakir, H. A. Kocak, H. Hammamci. The Construction And Use of Bead Mill For The Extraction Of Intracellular Proteins. Doga Tu Biolo.(Genetics, Microbiology, Molecular Biology, Cytology) Mar. 12, 1988, pp. 170-175.
W. Hummel and M. R. Kula. Simple Method For Small-Scale Disruption Of Bacteria And Yeasts. Journal Of Microbiological Methods (9), 1989, pp. 201-209.
S. Raha, F. Merante, G. Proteau and J. K. Reed. Simultaneous Isolation Of Total Cellular RNA and DNA From Tissue Culture Cells Using Phenol And Lithium Chloride. GATA 7(7), 1990, pp. 173-177.
Pritchard et al. "The Ultrasonic Degr
Melendez Luis A.
Olive D. Michael
Rosenbaum Robert E.
Swenson John E.
Vera-Garcia Marcela A.
Amoco Corporation
Beisner William H.
Tolpin Thomas
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